Mesp1 Antibody (2030B) [Unconjugated] - Azide Free

Western blot shows lysates of Mouse ES cells transfected with human MESP1 untreated (-) or treated (+) with 100 ng/mL Doxycycline overnight. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human MESP1 Monoclonal Antibody (Catalog # MAB9219) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MESP1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. Cells provided courtesy of Michael Kyba's lab, University of Minnesota.

Western blot shows lysates of H1 human embryonic stem cells untreated (-) or treated (+) with 100 ng/mL Doxycycline overnight. PVDF membrane was probed with Rabbit Anti-Human MESP1 Monoclonal Antibody (Catalog # MAB9219) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MESP1 at approximately 30 kDa (as indicated). Image provided courtesy of Michael Kyba's lab, University of Minnesota.

MESP1 was detected in fixed H1 human embryonic stem cells untreated (lower panel) or treated (upper panel) with Doxycycline using Rabbit Anti-Human MESP1 Monoclonal Antibody (Catalog # MAB9219). Cells were stained using anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).Image provided courtesy of Michael Kyba's lab, University of Minnesota.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 2030B
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

Mesp1 Antibody (2030B) [Unconjugated] - Azide Free Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-dervived recombinant human MESP1
  Met1-Gln85
  Accession # Q9BRJ9
• Specificity: Detects human MESP1 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MESP1
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL
• Application Notes: This antibody is Cytof ready.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mesp1 Antibody (2030B) [Unconjugated] - Azide Free

• bHLHc5
• bHLHc5MGC10676
• Class C basic helix-loop-helix protein 5
• mesoderm posterior 1 homolog (mouse)
• mesoderm posterior protein 1
• MESP1

Background

Mesoderm posterior protein 1 (MESP1) is a 268 amino acids protein that in humans is encoded by the MESP1 gene. MESP1 was first identified in transcripts enriched in the posterior region of the mouse embryo at embryonic day E7 to E7.5. Lineage tracing in mice showed that MESP1 represents the earliest marker of cardiac progenitors and directs multipotential cardiovascular cell fates, patterning mesoderm into cardiac, hematopoietic, or skeletal myogenic progenitors in a context-dependent manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mesp1 Antibody (MAB9219)(1)

Publication using MAB9219

• Jin G, Floy ME, Simmons AD et al. Spatial Stem Cell Fate Engineering via Facile Morphogen Localization Advanced healthcare materials 8/29/2021 [PMID: 34459150]

Bioinformatics

• Gene Symbol: MESP1
• Uniprot:
  • Q9BRJ9()