metallothionein Antibody (2B5) - BSA Free

Western Blot: metallothionein Antibody (2B5) [NBP3-18262] - Western Blot analysis of Pseudomonas aeruginosa metallothionein (PmtA) GST tagged showing detection of 36 kDa metallothionein protein using Mouse Anti-metallothionein Monoclonal Antibody, Clone 2B5 (NBP3-18262). Lane 1: Molecular Weight Ladder (MW). Lane 2: GST. Lane 3: Purified Pseudomonas aeruginosa metallothionein (PmtA) GST tagged. Load: 1 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-metallothionein Monoclonal Antibody (NBP3-18262) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 36 kDa.

• Reactivity: Ba
• Applications: WB, ELISA
• Clone: 2B5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

metallothionein Antibody (2B5) - BSA Free Summary

• Immunogen: Recombinant full length Pseudomonas aeruginosa Metallothionein protein
• Specificity: Detects 36 kDa band corresponding to Pseudomonas aeruginosa Metallothionein.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:800
  • Western Blot 1:1000
• Application Notes: Optimal dilutions for assays should be determined by the user.

Reactivity Notes

Bacteria (Pseudomonas aeruginosa)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS pH 7.4, 50% glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Background

Metallothioneins (MTs) are a class of ubiquitous, low molecular weight, cysteine-rich, metal binding proteins that function in the metabolism of zinc and copper. MT protects against exposure to toxic metals such as mercury and cadmium as well as a variety of other stress conditions. There are four mammalian MT isoforms: MT-I, -II, -III, -IV, each of which is encoded by individual genes. MT expression is induced by heavy metal cations, starvation, elevated temperature, lipopolysaccharide, inflammatory cytokines, free radicals, and ionizing radiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.