metallothionein Antibody (UC1MT) - BSA Free

Lane 1: MW marker, Lane 2: MT I, Lane 3: MT II, Lane 4: Mummichog CdCl2.

Human uterus tissue stained with Metallothionein, mAb (UC1MT) at 10ug/ml.

• Reactivity: Hu, Mu, Rt, Eq, Fi, Rb
• Applications: WB, ELISA, IHC
• Clone: UC1MT
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

metallothionein Antibody (UC1MT) - BSA Free Summary

• Immunogen: Cross-linked rabbit liver Metallothionein I and II.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:1000
• Application Notes: Immunohistochemistry-Paraffin and detects a band of ~15kDa by Western blot.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Background

Metallothioneins (MTs) are a class of ubiquitous, low molecular weight, cysteine-rich, metal binding proteins that function in the metabolism of zinc and copper. MT protects against exposure to toxic metals such as mercury and cadmium as well as a variety of other stress conditions. There are four mammalian MT isoforms: MT-I, -II, -III, -IV, each of which is encoded by individual genes. MT expression is induced by heavy metal cations, starvation, elevated temperature, lipopolysaccharide, inflammatory cytokines, free radicals, and ionizing radiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for metallothionein Antibody (NBP1-97493)(9)

Publications using NBP1-97493

• Tao Guo, Jian Wang, Xiangyu Meng, Ye Wang, Yihaoyun Lou, Jianglei Ma, Shuang Xu, Xiangyu Ni, Zongming Jia, Lichen Jin, Chengyu Wang, Qingyang Chen, Peng Li, Yuhua Huang, Shancheng Ren Deciphering the role of zinc homeostasis in the tumor microenvironment and prognosis of prostate cancer Discover Oncology 2024-06-04 [PMID: 38833013]
• Han H, Nakaoka HJ, Hofmann L et al. The Hippo pathway kinases LATS1 and LATS2 attenuate cellular responses to heavy metals through phosphorylating MTF1 Nature cell biology 2022-01-01 [PMID: 35027733]
• Kim JH, Jeon J, Shin M et al. Regulation of the Catabolic Cascade in Osteoarthritis by the Zinc-ZIP8-MTF1 Axis. Cell 2014-02-17 [PMID: 24529376] (IHC-P, Mouse)
• Escolar G, Diaz-Ricart M, Arellano-Rodrigo E, Gal�n AM. The pharmacokinetics of edoxaban for the prevention and treatment of venous thromboembolism. Expert opinion on drug metabolism & toxicology 2015-01-26 [PMID: 24471416]
• SG Kim et al. Potentiation of cadmium-induced cytotoxicity by sulfur amino acid deprivation through activation of extracellular signal-regulated kinase1/2 (ERK1/2) in conjunction with p38 kinase or c-jun N-terminal kinase (JNK). Complete inhibition of the potentiated t. Biochem. Pharmacol. 62, 1379. 2001-01-01 [PMID: 11709198] (WB, Rat)
• M Kantorow et al. Identification and spatial analysis of metallothioneins expressed by the adult human lens. Invest. Ophthalmol. Vis. Sci. 42, 188. 2001-01-01 [PMID: 11133866] (WB, Human)
• EA Olson et al. Immunomodulatory activities of extracellular metallothionein. I. Metallothionein effects on antibody production. Toxicology 85, 161 . 1993-01-01 [PMID: 8303711] (WB, Rabbit)
• Gumasta P Immunohistochemical Expression of Oxidative Stress Markers in Bovine Tissues Correlated to Cadmium Concentration JAR 2019-10-15
• Chun K, Lee C, Kim K et al. Effects of Androgen Receptor Inhibition on Kanamycin-Induced Hearing Loss in Rats International Journal of Molecular Sciences 2021-05-18 [PMID: 34070066] (WB, Rat)

FAQs for metallothionein Antibody (NBP1-97493). (Showing 1 - 3 of 3 FAQs).

1. Can you please confirm whether the following item can be recommended for IF?
   • I can confirm that this antibody has not been tested for use in IF. If you would be interested in testing this novel application, please take a look at our Innovators Reward Program.
2. Our customer would like to perform ELISA with NBP1-97493. Would you please help suggest the suitable protein product (Human preferred) to work with NBP1-97493 as the positive control or standard?
   • This antibody reacts to Metallothionein 1 and 2. Any of these Metallothionein 1 or 2 recombinant proteins should be suitable as a positive control.
3. Would you be able to give me details about how the example blot on the datasheet was run? (what percentage gel? what type of gel? what type of block?)
   • Indicated amounts of protein were denatured by boiling in 10% SDS buffer (10% w/v SDS; 0.5 M Tris-HCL, pH 6.8, 5% [v/v] 2-mercaptoethanol; 5% [v/v] glycerol), resolved by electrophoresis on 12% SDS-polyacrylamide gels and transferred (60 V for a half hour in 12 mM Tris-HCL, 96 mM glycine, 15% methanol) to nitrocellulose filters. The resulting blots were blocked overnight in Superblock (Pierce, Rock-ford, IL). The blot was washed three times with PBS over 15 minutes, incubated with a 1:200 dilution of mouse anti-MT antibody, and washed three times in PBS containing 0.1% Tween 20. Immunoreactive MT was visualized using ECL Western blotting reagents (Amersham-Pharmacia, Piscataway, NJ).