Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric)

ELISA: Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric) [NBP2-62161] - A typical standard curve for Methotrexate ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

• Reactivity: Hu, Mu, Rt
• Applications: ELISA
• Suitable Sample Type: Human, mouse, and rat serum, plasma, urine
• Standard Curve Range: 0.13-1000 ng/ml
• Sensitivity: 0.087 ng/ml

Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 0.13-1000 ng/ml
• Sensitivity: 0.087 ng/ml
• Assay Type: Competitive ELISA
• Inter-Assay: Low Concentration %CV - 22.4% High Concentration %CV - 17.1%
• Intra-Assay: Low Concentration %CV - 20.5% High Concentration %CV - 9.8%
• Spike Recovery: Human Serum Low - 71%
  Medium - 89%
  High - 86%
  
  Human Plasma Low - 91%
  Medium - 107%
  High - 77%
  
  Mouse Serum Low - 141%
  Medium - 140%
  High - 135%
  
  Mouse Plasma Low - 90%
  Medium - 168%
  High - 56%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Alternate Names for Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric)

• (+)-Amethopterin
• 4-amino-10-methylfolic acid
• 4-Amino-N10-methylpteroylglutamic Acid
• 59-05-2
• Amethopterine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.