mGluR5/1 Antibody (ST51-00)

Western Blot: mGluR5/1 Antibody (ST51-00) [NBP2-67508] - Western blot analysis of mGluR5/1 on PC-12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti

Immunocytochemistry/Immunofluorescence: mGluR5/1 Antibody (ST51-00) [NBP2-67508] - Staining GRM5 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: mGluR5/1 Antibody (ST51-00) [NBP2-67508] - Analysis of paraffin-embedded mouse brain tissue using anti-GRM5 antibody. Counter stained with hematoxylin.

Flow Cytometry: mGluR5/1 Antibody (ST51-00) [NBP2-67508] - Analysis of SH-SY-5Y cells with GRM5 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: mGluR5/1 Antibody (ST51-00) [NBP2-67508] - Staining GRM5 in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: mGluR5/1 Antibody (ST51-00) [NBP2-67508] - Staining GRM5 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: mGluR5/1 Antibody (ST51-00) [NBP2-67508] - Analysis of paraffin-embedded rat brain tissue using anti-GRM5 antibody. Counter stained with hematoxylin.

Product Discontinued

mGluR5/1 Antibody (ST51-00) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human mGluR5/1 aa 1,163-1,212 / 1,212. (SwissProt: P41594 Human; SwissProt: Q3UVX5 Mouse; SwissProt: P31424 Rat)
• Localization: Cell membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GRM5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for mGluR5/1 Antibody (ST51-00)

• glutamate receptor, metabotropic 5
• GPRC1EMGLUR5metabotropic glutamate receptor 5
• mGlu5
• mGluR5

Background

Glutamate is the main excitatory neurotransmitter in the brain. For many years it had been considered to act only on the ligand-gated receptor channels-termed NMDA, AMPA and kainate receptors that are involved in the fast excitatory synaptic transmission. Recently, glutamate has been shown to regulate enzymes producing second messengers via specific receptors coupled to G-proteins. These receptors are called metabotropic glutamate receptors. In expression systems, Group-I receptors, stimulate phospholipase C as revealed by an increase in phosphoinositide turnover, and Ca2+ release from internal stores. Group-II and -III receptors are coupled to the inhibition of adenylyl cyclase. The Group-I receptors include mGluR1a and mGluR5. The Group-II receptors in include mGluR2 and mGluR3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRM5