MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free

Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [NBP1-43312] - Analysis using the PE conjugate of NBP1-43312. Analysis of mouse splenocyte suspensions using MHC Class II PE and can be used at less than or equal to 0.125 ug per test. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [NBP1-43312] - Staining of C57BL/6 splenocytes with 0.06 ug of Rat IgG2b K Isotype Control Purified (cat. NBP1-43323) (open histogram) or 0.06 ug of Anti-Mouse MHC Class II (I-A/I-E) Purified (filled histogram) followed by Anti-Rat IgG FITC (cat. NBP1-69751). Total viable cells were used for analysis.

Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [NBP1-43312] - Analysis using the Allophycocyanin conjugate of NBP1-43312. Staining of C57BL/6 splenocytes with Anti-Mouse CD3e FITC and 0.015 micrograms of Rat IgG2b ? Isotype Control APC (left) or 0.015 micrograms of Anti-Mouse MHC Class II (I-A/I-E) APC (right).

Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [NBP1-43312] - Analysis using the Biotin conjugate of NBP1-43312. Staining of C57BL/6 splenocytes with 0.015 ug of Rat IgG2b K Isotype Control Biotin (open histogram) or 0.015 ug of Anti-Mouse MHC Class II (I-A/I-E) Biotin followed by Streptavidin PE (filled histogram).

Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [NBP1-43312] - Analysis using the FITC conjugate of NBP1-43312. Staining of C57BL/6 splenocytes with Anti-Mouse CD3e and 0.06 micrograms of Rat IgG2b k Isotype Control FITC (left) or 0.06 micrograms of Anti-Mouse MHC Class II (I-A/I-E) FITC (right). Cells in the lymphocyte gate were used for analysis.

• Reactivity: Hu, Mu
• Applications: WB, Flow, IHC, IP
• Clone: M5/114.15.2
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free Summary

• Immunogen: The immunogen for this antibody was MHC Class II.
• Specificity: The monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: H2-Ea
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry < 0.125 ug/10^6 cells
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: The M5/114.15.2 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions and can be used at less than or equal to 0.125 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Reactivity Notes

Use in Human reported in scientific literature (PMID:29990503).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free

• E-alpha-f;H-2 class II histocompatibility antigen, E-K alpha chain;H-2 class II histocompatibility antigen, E-U alpha chain;H-2E;H2-Ea;H-2Ea;H2-Ea-ps;I;Ia;Ia3;Ia-3;I-E;I-Ealpha;MHC-H2-Ea
• H2-Ea
• MHC class II (I-A/I-E)
• MHC class II IA/IE

Background

The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u but not from mice carrying the H-2s or H-2f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, or H-2s haplotypes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MHC class II (I-A/I-E) Antibody (NBP1-43312)(23)

Publications using NBP1-43312

• Gallage S, Ali A, Barragan Avila JE, Seymen N et Al. A 5:2 intermittent fasting regimen ameliorates NASH and fibrosis and blunts HCC development via hepatic PPAR? and PCK1 Cell Metab 2024-05-08 [PMID: 38718791]
• Luckett, T;Abudula, M;Ireland, L;Glenn, M;Bellomo, G;Stafferton, R;Halloran, C;Ghaneh, P;Jones, R;Schmid, MC;Mielgo, A; Mesothelin Secretion by Pancreatic Cancer Cells Co-opts Macrophages and Promotes Metastasis Cancer research 2024-02-15 [PMID: 38356443]
• Funk MC, Gleixner JG, Heigwer F, Vonficht D et Al. Aged intestinal stem cells propagate cell-intrinsic sources of inflammaging in mice Dev Cell 2023-12-19 [PMID: 38113852]
• Nithianandam V, Bukhari H, Leventhal M et al. Integrative analysis reveals a conserved role for the amyloid precursor protein in proteostasis during aging bioRxiv 2023-10-09 [PMID: 37923712]
• Xiao J, Huang J, Jian X et al. IRE1? arm of unfolded protein response in muscle-specific TGF-? signaling-mediated regulation of muscle cell immunological properties Cellular & molecular biology letters 2023-02-27 [PMID: 36849929] (WB, Mouse)
• Tanaka T, Okuda H, Isonishi A et al. Dermal macrophages set pain sensitivity by modulating the amount of tissue NGF through an SNX25-Nrf2 pathway Nature immunology 2023-01-26 [PMID: 36703006] (IHC-Fr, Mouse)
  
  Details:
  Dilution used 1:500
• Balmert SC, Ghozloujeh ZG, Carey CD et al. A microarray patch SARS-CoV-2 vaccine induces sustained antibody responses and polyfunctional cellular immunity iScience 2022-10-21 [PMID: 36062075]
• Sobel RA, Albertelli M, Hinojoza JR et al. Azetidine-2-Carboxylic Acid-Induced Oligodendrogliopathy: Relevance to the Pathogenesis of Multiple Sclerosis Journal of Neuropathology & Experimental Neurology 2022-05-20 [PMID: 35521963]
• AK Rohlfing, K Kolb, M Sigle, M Ziegler, A Bild, P Münzer, J Sudmann, V Dicenta, T Harm, MC Manke, S Geue, M Kremser, M Chatterjee, C Liang, H von Eysmon, T Dandekar, D Heinzmann, M Günter, S von Ungern, M Büttcher, T Castor, S Mencl, F Langhauser, K Sies, D Ashour, MC Beker, M Lämmerhofe, SE Autenrieth, TE Schäffer, S Laufer, P Szklanna, P Maguire, M Heikenwald, KAL Müller, DM Hermann, E Kilic, R Stumm, G Ramos, C Kleinschni, O Borst, HF Langer, D Rath, M Gawaz ACKR3 regulates platelet activation and ischemia-reperfusion tissue injury Nature Communications, 2022-04-05;13(1):1823. 2022-04-05 [PMID: 35383158]
• Deczkowska A, David E, Ramadori P et al. XCR1+ type 1 conventional dendritic cells drive liver pathology in non-alcoholic steatohepatitis Nature medicine 2021-05-20 [PMID: 34017133]
• Simion V, Zhou H, Haemmig S et al. A macrophage-specific lncRNA regulates apoptosis and atherosclerosis by tethering HuR in the nucleus Nature communications 2020-12-01 [PMID: 33262333] (IHC-Fr, Mouse)
• Pires, A, Greenshields-Watson, A Et al. Immune Remodeling of the Extracellular Matrix Drives Loss of Cancer Stem Cells and Tumor Rejection. Cancer Immunol Res 2020-12-01 [PMID: 33023965] (IHC-Fr, Mouse)
• Feldmann K, Maurer C, Peschke K et al. Mesenchymal plasticity regulated by Prrx1 drives aggressive pancreatic cancer biology Gastroenterology 2020-09-29 [PMID: 33007300] (IF/IHC, Mouse)
• Hoefflin R, Harlander S, SchAfer S et al. HIF-1 alpha and HIF-2 alpha differently regulate tumour development and inflammation of clear cell renal cell carcinoma in mice Nat Commun 2020-01-01 [PMID: 32807776] (IF/IHC, Mouse)
• Loffredo-Verde E, Bhattacharjee S, Malo A, et al. Dynamic, Helminth-Induced Immune Modulation Influences the Outcome of Acute and Chronic Hepatitis B Virus Infection J. Infect. Dis. 2019-12-25 [PMID: 31875228] (IF/IHC, Mouse)
• Guerrero A, Herranz N et al. Cardiac glycosides are broad-spectrum senolytics. Nat Metab 2019-01-11 [PMID: 31799499] (FLOW, Mouse)
• O'Connor T, Zhou X, Kosla J et al. Age-Related Gliosis Promotes Central Nervous System Lymphoma through CCL19-Mediated Tumor Cell Retention Cancer Cell 2019-09-16 [PMID: 31526758] (Mouse)
• Seehawer M, Heinzmann F, D'Artista L et al. Necroptosis microenvironment directs lineage commitment in liver cancer. Nature 2018-09-12 [PMID: 30209397] (IHC-P, Mouse)
• Georgilis A, Klotz S et al. PTBP1-Mediated Alternative Splicing Regulates the Inflammatory Secretome and the Pro-tumorigenic Effects of Senescent Cells. Cancer Cell. Sep 7 2018 [PMID: 29990503] (IHC, Human)
• Georgilis A, Klotz S et al. PTBP1-Mediated Alternative Splicing Regulates the Inflammatory Secretome and the Pro-tumorigenic Effects of Senescent Cells. Cancer Cell Sep 7 2018 [PMID: 29990503] (IHC, Human)
• Matsuzaka Y, Tanihata J, Komaki H et al. Characterization and Functional Analysis of Extracellular Vesicles and Muscle-Abundant miRNAs (miR-1, miR-133a, and miR-206) in C2C12 Myocytes and mdx Mice. PLoS ONE. 2016-12-15 [PMID: 27977725] (IP, Mouse)
• Gallage S, Ali A, Barragan Avila J et al. Beneficial effects of intermittent fasting in NASH and subsequent HCC development are executed by concerted PPAR? and PCK1 action in hepatocytes bioRxiv 2023-10-25 (IHC-P, Mouse)
  
  Details:
  Dilution 1:250
• Huang LR, Wohlleber D, Reisinger F et al. Intrahepatic myeloid-cell aggregates enable local proliferation of CD8+ T cells and successful immunotherapy against chronic viral liver infection. Nat Immunol 2013-04-14 [PMID: 23584070] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: H2-Ea