MHC class II (I-A/I-E) Antibody (M5/114.15.2) [PE/Cy5.5]

• Reactivity: Hu, Mu
• Applications: Flow
• Clone: M5/114.15.2
• Clonality: Monoclonal
• Host: Rat
• Conjugate: PE/Cy5.5

MHC class II (I-A/I-E) Antibody (M5/114.15.2) [PE/Cy5.5] Summary

• Immunogen: The immunogen for this antibody was MHC Class II.
• Specificity: The monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: H2-Ea
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Reactivity Notes

Use in Human reported in scientific literature (PMID:29990503).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for MHC class II (I-A/I-E) Antibody (M5/114.15.2) [PE/Cy5.5]

• E-alpha-f;H-2 class II histocompatibility antigen, E-K alpha chain;H-2 class II histocompatibility antigen, E-U alpha chain;H-2E;H2-Ea;H-2Ea;H2-Ea-ps;I;Ia;Ia3;Ia-3;I-E;I-Ealpha;MHC-H2-Ea
• H2-Ea
• MHC class II (I-A/I-E)
• MHC class II IA/IE

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H2-Ea