Midkine Antibody (JF096-5)

Knockout Validated: Midkine Antibody (JF096-5) [NBP2-66948] - ~30 ug of protein from concentrated culture media were processed for immunoblot with the Midkine antibody (JF096-5). The Ponceau stained transfer of the blot is shown. Antibody dilution used: 1/500. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Immunohistochemistry-Paraffin: Midkine Antibody (JF096-5) [NBP2-66948] - Analysis of paraffin-embedded human pancreas tissue using anti-Midkine antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Midkine Antibody (JF096-5) [NBP2-66948] - Analysis of paraffin-embedded human liver cancer tissue using anti-Midkine antibody. Counter stained with hematoxylin.

Immunoprecipitation: Midkine Antibody (JF096-5) [NBP2-66948] - lmmunoprecipitation was performed on concentrated culture media using 1.0 ug of Midkine antibody (JF096-5). Sample was washed and processed for immunoblot with Midkine antibody (JF096-5). For immunoblot, NBP2-66948 was used at 1/500. The Ponceau stained transfer of the blot is shown. SM=10% starting material; UB=10% unbound fraction; IP=immunoprecipitate. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Product Discontinued

Midkine Antibody (JF096-5) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Midkine aa 100-143 / 143. (SwissProt: P21741 Human)
• Localization: Secreted.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MDK
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Knockout Validated
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Midkine Antibody (JF096-5)

• Amphiregulin-associated protein
• ARAP
• MDK
• MEK
• Midgestation and kidney protein
• midkine (neurite growth-promoting factor 2)
• Midkine
• MK1
• MKARAP
• NEGF2
• NEGF2FLJ27379
• Neurite outgrowth-promoting factor 2
• Neurite outgrowth-promoting protein

Background

Midkine (MK) is a 13 kDa heparin-binding polypeptide which enhances neurite outgrowth, neuronal cell survival and plasminogen activator activity. MK is structurally divided into two domains, and most of the biological activities are located on the C-terminal domain. Both domains consist of three antiparallel beta-strands, but the C-terminal domain has a long flexible hairpin loop where a heparin-binding consensus sequence is located (1). MK is a member of a highly conserved, developmentally regulated human gene family. The gene product exhibits neurite outgrowth-promoting activity and may play a role in nervous system development and/or maintenance. Expression of MK is predominant only for a short period from approximately one-half to two-thirds of the way through gestation; before and after that, it is barely detectable (2)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MDK