Recombinant Rat MIF Protein

SDS-Page: Rat MIF Protein [NBP2-35276]

• Reactivity: Rt
• Applications: PAGE
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Rat MIF Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 115 amino acids corresponding to MIF Source: E. coli

  Uniprot ID: P30904

  Amino Acid Sequence: MPMFIVNTNV PRASVPEGFL SELTQQLAQA TGKPAQYIAV HVVPDQLMTF SGTSDPCALC SLHSIGKIGG AQNRNYSKLL CGLLSDRLHI SPDRVYINYY DMNAANVGWN GSTFA

• Details of Functionality: Test in process
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: MIF
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of MIF as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 12.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris, pH 8.0, 150 mM NaCl, 3 % trehalose.
• Preservative: Trehalose
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Rat MIF Protein

• EC 5.3.2.1
• EC 5.3.3.12
• GIFmacrophage migration inhibitory factor
• GLIF
• Glycosylation-inhibiting factor
• L-dopachrome isomerase
• L-dopachrome tautomerase
• macrophage migration inhibitory factor (glycosylation-inhibiting factor)
• MIF
• MMIF
• Phenylpyruvate tautomerase

Background

Macrophage migration inhibitory factor (MIF or MMIF), also named as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase, is a protein encoded by the MIF gene. It is released from white blood cells by bacterial antigen stimulation to trigger an acute immune response, or by glucocorticoids to counter-act the inhibitory effects of glucocorticoids on immune system. MIF is a homotrimer of which each subunit contains 115 amino acids. As mentioned above, MIF is involved in the innate immune response to bacterial pathogens and counter-acts the anti-inflammatory activity of glucocorticoids. Furthermore, it also plays a role as mediator in regulating the function of macrophages in host defense and has phenylpyruvate tautomerase and dopachrome tautomerase activity in vitro. Rat MIF is 99 %, 90 %, 89 %, and 89 % a.a. identical to human, murine, porcine and bovine, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for MIF Protein (NBP2-35276)(1)

Publication using NBP2-35276

• Liu J, Chen Y, Chen H et al. Macrophage migration inhibitory factor exacerbates asthmatic airway remodeling via dynamin-related protein 1-mediated autophagy activation Respiratory research 2023-09-06 [PMID: 37674165] (, In vitro assay)

FAQs for MIF Protein (NBP2-35276). (Showing 1 - 1 of 1 FAQ).

1. Could we use this protein for in vivo assay and cell culture (supplements)?
   • This is a recombinant protein expressed in E. coli. Therefore it is not properly glycosylated, if at all. However, it has been tested for the levels of the endotoxin: Less than 1 EU/ug of endotoxin as determined by LAL method. In this case, this may be used in the live culture (in vivo), without eliciting any unwanted side effects.

Bioinformatics

• Gene Symbol: MIF