MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free

Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Kindlin-2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Kindlin-2.

Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Kindlin-2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Kindlin-2.

Immunohistochemistry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Staining of paraffin-embedded Human pancreas tissue using anti-Kindlin-2 mouse monoclonal antibody.

Flow Cytometry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Staining of HepG2 cells using anti-Kindlin-2 mouse monoclonal antibody.

Western Blot: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Kindlin-2 monoclonal antibody.

Immunohistochemistry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Kindlin-2 mouse monoclonal antibody.

Immunohistochemistry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Kindlin-2 mouse monoclonal antibody.

Immunohistochemistry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Kindlin-2 mouse monoclonal antibody.

Immunohistochemistry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Kindlin-2 mouse monoclonal antibody.

Immunohistochemistry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Staining of paraffin-embedded Human colon tissue using anti-Kindlin-2 mouse monoclonal antibody.

Immunohistochemistry: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - Staining of paraffin-embedded Human Ovary tissue using anti-Kindlin-2 mouse monoclonal antibody.

Immunoprecipitation: MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free [NBP2-72687] - (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FERMT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: OTI9E4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free Summary

• Immunogen: Full-length protein expressed in 293T cell transfected with human FERMT2 expression vector
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: FERMT2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 2ug/500ul
  • Western Blot 1:500-1000
• Theoretical MW: 77.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for MIG2/Kindlin-2 Antibody (OTI9E4) - Azide and BSA Free

• fermitin family homolog 2 (Drosophila)
• fermitin family homolog 2
• fermitin family member 2
• FERMT2
• KIND2
• KIND2DKFZp686G11125
• kindlin 2
• Kindlin-2
• MIG2
• mig-2
• MIG2FLJ34213
• mitogen inducible gene 2 protein
• Mitogen-inducible gene 2 protein
• PH domain-containing family C member 1
• pleckstrin homology domain containing, family C (with FERM domain) member 1
• pleckstrin homology domain containing, family C member 1
• Pleckstrin homology domain-containing family C member 1
• PLEKHC1
• PLEKHC1FLJ44462
• UNC112
• UNC112B

Background

Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FERMT2