Mitochondria Antibody (113-1)

Western Blot: Mitochondria Antibody (113-1) [NBP2-32980] - Western Blot analysis of HeLa cell lysate using Mitochondria Antibody (113-1).

Immunocytochemistry/Immunofluorescence: Mitochondria Antibody (113-1) [NBP2-32980] - Confocal immunofluorescence image of HeLa cells using labeled is Green (CF488) and Red Dot is used to label the nuclei Red.

Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) [NBP2-32980] - Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) - Azide and BSA Free [NBP2-34517] - Formalin-fixed paraffin-embedded human Renal cell carcinoma stained with Mitchondria Monoclonal antibody (113-1). Image using the Azide and BSA Free form of this antibody.

Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) [NBP2-32980] - Analysis using the Biotin conjugate of NBP2-32980. Staining of human prostate cancer tissue (left) and mouse stomach tissue (right). Image from verified customer review.

Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) [NBP2-32980] - Formalin-fixed, paraffin-embedded human testicular carcinoma stained with mitochondria monoclonal antibody (113-1). Image using the Azide and BSA Free form of this antibody.

Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) [NBP2-32980] - Formalin-fixed paraffin-embedded human pancreas stained with mitochondria monoclonal antibody (113-1). Image using the Azide and BSA Free form of this antibody.

Immunocytochemistry/Immunofluorescence: Mouse Monoclonal Mitochondria Antibody (113-1) [NBP2-32980] - NBP2-32980 (green) stains mitochondria in human primary astrocytes. Cells have been counterstained with Hoechst (blue). Image from a verified customer review.

• Reactivity: Hu, Mu(-), Rt(-)
• Applications: WB, ICC/IF, IHC
• Clone: 113-1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Mitochondria Antibody (113-1) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-34517)
  
  Antibody with azide - store at 2 to 8C.
• Immunogen: Semi-Purified mitochondrial preparation
• Localization: Mitochondria in cytoplasm
• Marker: Marker for Human Cells, Granular RCC s & Salivary Tumor
• Specificity: This monoclonal antibody recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells. Immunostaining pattern with anti-mitochondrial monoclonal antibody has been reported as a useful discriminatory adjunct in the complex differential diagnosis of granular renal cell tumors. Reportedly, this monoclonal antibody facilitates the classification of salivary tumors.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Does not react with Mouse or Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Background

NB600-556 recognizes a 60kDa non-glycosylated protein component of mitochondria found in human cells. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a marker of the mitochondria in subcellular fractions. This monoclonal antibody is part of a new panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb-2 produces a spaghetti-like staining pattern in the cytoplasm of normal and malignant human cells and may be used to stain the mitochondria in paraformaldehyde fixed frozen and formalin-fixed, paraffin-embedded tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mitochondria Antibody (NBP2-32980)(1)

Publication using NBP2-32980

• Chang TH, Shanti RM, Liang Y et al. LGR5+ epithelial tumor stem-like cells generate a 3D-organoid model for ameloblastoma Cell Death Dis 2020-05-07 [PMID: 32382005] (IHC-Fr, Human)

Review for Mitochondria Antibody (NBP2-32980) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Pauline Jeannot IF Human 08/21/2024

Summary

• Application: Immunofluorescence
• Sample Tested: primary astrocytes
• Species: Human
• Lot: D162766