Mitochondria Antibody (MTC02)

Immunohistochemistry-Paraffin: Mitochondria Antibody (MTC02) [NBP2-32982] - Formalin-fixed, paraffin-embedded human Pancreas stained with Mitochondria Antibody (MTC02).

Immunohistochemistry-Paraffin: Mitochondria Antibody (MTC02) [NBP2-32982] - Formalin-fixed, paraffin-embedded human Tonsil stained with Mitochondria Monoclonal Antibody (MTC02).

• Reactivity: Hu, Rt(-)
• Applications: ICC/IF, IHC
• Clone: MTC02
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Mitochondria Antibody (MTC02) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-34686)
  
  Antibody with azide - store at 2 to 8C.
• Immunogen: Semi-Purified mitochondrial preparation
• Localization: Mitochondria in cytoplasm
• Marker: Marker for Human Cells
• Specificity: This monoclonal antibody recognizes a 60kDa antigen associated with the mitochondria in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This monoclonal antibody recognizes an antigen associated with the mitochondria in human cells only. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:200-1:400
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 1mM EDTA Buffer, pH 8.5-9.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Does not react with Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Background

NB600-556 recognizes a 60kDa non-glycosylated protein component of mitochondria found in human cells. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a marker of the mitochondria in subcellular fractions. This monoclonal antibody is part of a new panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb-2 produces a spaghetti-like staining pattern in the cytoplasm of normal and malignant human cells and may be used to stain the mitochondria in paraformaldehyde fixed frozen and formalin-fixed, paraffin-embedded tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mitochondria Antibody (NBP2-32982)(4)

Publications using NBP2-32982

• Moreno S, Marioli C, Muzzi M et al. Caspase-dependent apoptosis in Riboflavin Transporter Deficiency iPSCs and derived motor neurons Research Square 2023-06-29 (ICC/IF, Human)
  
  Details:
  1:200 dilution
• Nagasawa Y, Takei M, Iwata M et al. Human osteoclastogenesis in Epstein-Barr virus-induced erosive arthritis in humanized NOD/Shi--scid/IL-2R gamma null mice PloS one 2021-04-01 [PMID: 33793647] (ICC/IF, Mouse)
• Boopathy AV, George A, Trac D et al. Age-Dependent Effect of Pediatric Cardiac Progenitor Cells After Juvenile Heart Failure. Stem Cells Transl Med. 2016-07-01 [PMID: 27151913] (IHC-P, Human)
• Cueno ME, Saito Y, Ochiai K. Periodontal disease level-butyric acid amounts locally administered in the rat gingival mucosa induce ER stress in the systemic blood. Microb. Pathog. 2015-11-02 [PMID: 26541671]