Mitochondrial DNA Isolation DNA Purification Kit

Mitochondrial DNA Isolation DNA Purification Kit [KA0895] - Mitochondrial DNA was isolated from HeLa cells (5 x 106) and rat liver (~50 mg) according to the kit protocol. DNA was measured using Nanodrop at 260 nm.

Product Discontinued

Mitochondrial DNA Isolation DNA Purification Kit Summary

• Description: Mitochondrial DNA Isolation Kit is used for isolating mtDNA from a variety of cells.
• Kit Type: DNA Purification Kit

Applications/Dilutions

• Dilutions:
  • Functional
• Application Notes: Useful in DNA isolation and Genome Sequencing.

Reactivity Notes

Mammal mitochondrial DNA. Suitable on mammal cells and tissues. Reacts with Dragonfly.

Packaging, Storage & Formulations

• Storage: Store at -20C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Background

Mitochondria are semiautonomous organelles which function in the aging process, apoptosis, anti-HIV drugs, and cancers. Mitochondrial DNA (mtDNA) has a very high mutation rate, and these mutations appear to be related to certain diseases such as diabetes, Alzheimer's disease, and muscle disorders. Isolation and quantification of mtDNA is often required to study the relationships between the diseases and mtDNA. This Mitochondrial DNA Isolation Kit provides convenient tools for isolating mtDNA from a variety of cells and tissues in high yield and purity, without contaminations from genomic DNA. The purified mtDNA can be used for a variety of studies such as enzyme manipulations, Southern blotting, cloning, PCR analysis, and amplifications

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Mitochondrial DNA Isolation Kit (KA0895)(8)

Publications using KA0895

• Yong HS, Song SL, Lim PE et al. Complete Mitochondrial Genome of Three Bactrocera Fruit Flies of Subgenus Bactrocera (Diptera: Tephritidae) and Their Phylogenetic Implications. PLoS One 2016-02-03 [PMID: 26840430]
• Gao X, Campian JL, Qian M et al. Mitochondrial DNA damage in iron overload. J Biol Chem. 2009-02-20 [PMID: 19095657]
• Hoi-Sen Yong HS, Song SL, Lim PE et al. Complete mitochondrial genome of Eurema blanda and phylogenetic relationship with its congener E. hecabe and other pierid butterflies. Meta Gene 2016-08-12 (Insect)
• Hoi-Sen Yong HS, Song SL, Lim PE et al. Complete mitochondrial genome of Eurema blanda and phylogenetic relationship with its congener E. hecabe and other pierid butterflies. Meta Gene Aug 12 2016
• Yong HS, Song SL, Suana IW et al. Complete mitochondrial genome of Orthetrum dragonflies and molecular phylogeny of Odonata. Biochemical Systematics and Ecology Sep 10 2016
• Yong HS, Song SL, Lim PE et al. Complete mitochondrial genome and phylogeny of Microhyla butleri (Amphibia: Anura: Microhylidae). Mitochondrial DNA B Resour 2021-01-21 [PMID: 33473443]
• Yuan G, Yang S, Liu M, Yang S RGS12 is required for the maintenance of mitochondrial function during skeletal development Cell discovery 2020-09-01 [PMID: 32922858]
• Schmid JA, Berti M, Walser F et al. Histone Ubiquitination by the DNA Damage Response Is Required for Efficient DNA Replication in Unperturbed S Phase. Mol Cell 2018-08-16 [PMID: 30122534]

Review for Mitochondrial DNA Isolation Kit (KA0895) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Wen Zhang Flow Other 10/06/2014

Summary

• Application: Flow Cytometry
• Sample Tested: DNA plasmid from E coli expression
• Species: Other