MMP-1 Antibody (6A5) - BSA Free

Western Blot: MMP-1 Antibody (6A5) [NBP2-22123] - Western blot analysis using MMP1 mAb against HEK293 (1) and MMP1(AA: 24-213)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: MMP-1 Antibody (6A5) [NBP2-22123] - Immunofluorescence analysis of Hela cells using MMP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: MMP-1 Antibody (6A5) [NBP2-22123] - Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues (left) and human kidney cancer tissues (right) using MMP1 mouse mAb with DAB staining.

Flow Cytometry: MMP-1 Antibody (6A5) [NBP2-22123] - Flow cytometric analysis of Hela cells using MMP1 mouse mAb (green) and negative control (purple).

ELISA: MMP-1 Antibody (6A5) [NBP2-22123] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 6A5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

MMP-1 Antibody (6A5) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human MMP1 expressed in E. coli. [UniProt# P03956]
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MMP1
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Application Notes: This MMP1 (6A5) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry and ELISA.
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Other species have not been tested.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for MMP-1 Antibody (6A5) - BSA Free

• CLGmatrix metalloprotease 1
• CLGN
• EC 3.4.24
• EC 3.4.24.7
• Fibroblast collagenase
• interstitial collagenase
• matrix metallopeptidase 1 (interstitial collagenase)
• matrix metalloproteinase 1 (interstitial collagenase)
• Matrix metalloproteinase-1
• MMP1
• MMP-1

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMP-1 Antibody (NBP2-22123)(5)

Publications using NBP2-22123

• Tran, TT;Lee, G;Huh, YH;Chung, KH;Lee, SY;Park, KH;Kwon, SH;Kook, MS;Chun, JS;Koh, JT;Ryu, JH; Disruption of cholesterol homeostasis triggers periodontal inflammation and alveolar bone loss Experimental & molecular medicine 2023-12-01 [PMID: 38036731]
• Su-Jin Kim, Se Hui Lee, Binh Do Quang, Thanh-Tam Tran, Young-Gwon Kim, Jun Ko, Weon-Young Choi, Sun Young Lee, Je-Hwang Ryu Avenanthramide-C Shows Potential to Alleviate Gingival Inflammation and Alveolar Bone Loss in Experimental Periodontitis Molecules and Cells 2023-10-31 [PMID: 37641936]
• Zhou Y, Song Z, Hu Q et al Evaluation of the Expression of Matrix Metalloproteinase-1 of Laryngeal Squamous Cell Carcinoma by Ultrasound Molecular Imaging. Front Pharmacol. 2019-06-19 [PMID: 31275143] (ICC/IF, Mouse)
  
  Details:
  Citation using the Biotin version of this antibody.
• Gharbia S, Balta C, Herman H et al. Enhancement of Silymarin Anti-fibrotic Effects by Complexation With Hydroxypropyl (HPBCD) and Randomly Methylated (RAMEB) b-Cyclodextrins in a Mouse Model of Liver Fibrosis Front Pharmacol 2018-08-13 [PMID: 30150935] (IHC-P, Mouse)
• Kim MW, Park JT, Kim JH et al. Periostin in Mature Stage Localized Scleroderma.. Ann Dermatol 2017-06-01 [PMID: 28566901] (IF/IHC, Human)

Review for MMP-1 Antibody (NBP2-22123) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Shinford Sun ICC Human 08/11/2023

Summary

• Application: Immunocytochemistry
• Sample Tested: fibroblasts,human fibroblast cells and mouse liver tissue, Sample Amount: 100 ug,Human lung cancer tissue samples
• Species: Human
• Lot: A-6

Bioinformatics

• Gene Symbol: MMP1
• Entrez:
  • 4312(Human)
• Uniprot:
  • P03956()