MMR/CD206/Mannose Receptor Antibody (857615) [Unconjugated]

J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse MMR/CD206 Monoclonal Antibody (Catalog # MAB25351, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 857615
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

MMR/CD206/Mannose Receptor Antibody (857615) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse MMR
  Leu19-Ala1388
  Accession # Q61830
• Specificity: Detects mouse MMR in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: MRC1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMR/CD206/Mannose Receptor Antibody (857615) [Unconjugated]

• CD206
• CLEC13D
• CLEC13Dmacrophage mannose receptor 1
• C-type lectin domain family 13 member D
• mannose receptor, C type 1
• MMR
• MMRCD206 antigen
• MRC1

Background

The MMR (macrophage mannose receptor) is also called MR due to its presence on cells other than macrophages, and is designated CD206, Mrc1 (mannose receptor C type 1), or CLEC13D (C‑type lectin domain family 13, member D) (1‑4). CD206 is a 175 kDa endocytic receptor that is expressed on M2 alternatively activated tissue macrophages including tumor‑associated macrophages (TAMs), inflammatory dendritic cells in selected lymphoid organs, and liver, splenic, lymphatic, and dermal microvascular endothelial cells (1, 2, 5‑8). The 1456 amino acid (aa) mouse CD206 precursor contains a signal sequence (19 aa), an extracellular domain (ECD) containing an N‑terminal cysteine‑rich domain, a fibronectin type II repeat, eight C‑type lectin domains (CTLDs), and several N‑glycosylation sites (1369 aa), a transmembrane segment and a short (47 aa) cytoplasmic domain (2‑4). Metalloproteinases can mediate the shedding of the soluble ECD (2). The mouse CD206 ECD shares 96% aa sequence identity with rat MR, and 83‑84% with human, equine, porcine and canine CD206. The cysteine-rich domain recognizes some pituitary hormones such as LH (luteinizing hormone/lutropin) and TSH (thyroid stimulating hormone/thyrotropin), chondroitin sulfates, and sulfated N‑acetylgalactosamines including sulfo‑Lewis^a and ‑Lewis^x (1, 7, 9). The FNII domain mediates Ca2⁺‑independent binding of collagens (2, 10). The CTLDs participate in Ca2⁺‑dependent recognition of branched sugars with terminal mannose, fucose or N‑acetylglucosamine that occur on many pathogenic microorganisms (7, 11). CD206 internalizes ligands in clathrin-coated vesicles, sorts them to phagosomes or early endosomes, and recycles to the cell surface (1, 6, 7). CD206 also promotes clearance of glycoproteins that promote allergy or ongoing inflammation, such as lysosomal hydrolases and myeloperoxidases (1, 2, 5‑7). It is involved in T cell polarization and production of pro‑ and anti‑inflammatory cytokines (1, 2). It facilitates peptide presentation on MHC II, and cross‑presentation on MHC I which is important for tumor immunogenicity (1, 2, 12). This function may be blocked by engagement of CD206 on TAMs by tumor mucins (8).

1. Gazi, U. and L. Martinez-Pomares (2009) Immunobiology 214:554.
2. Martinez-Pomares, L. (2012) J. Leukoc. Biol. 92:1177.
3. Harris, N. et al. (1992) Blood 80:2363.
4. Taylor, M.E. et al. (1990) J. Biol. Chem. 265:12156.
5. Chieppa, M. et al. (2003) J. Immunol. 171:4552.
6. Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77.
7. Taylor, P.R. et al. (2005) Trends Immunol. 26:104.
8. Allavena, P. et al. (2010) Clin. Dev. Immunol. 2010:547179.
9. Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
10. Martinez-Pomares, L. et al. (2006) Eur. J. Immunol. 36:1074.
11. Taylor, M.E. et al. (1992) J. Biol. Chem. 267:1719.
12. Singh S.K. et al. (2011) Eur. J. Immunol. 41:916.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMR/CD206/Mannose Receptor Antibody (MAB25351)(1)

Publication using MAB25351

• Choong-kun Lee, Bo-Yoon Park, Taehee Jo, Cheol-Heum Park, Ju-Hee Kim, Kyu-Jin Chung et al. Dll4 Inhibition Promotes Graft Retention in Fat Grafting Enriched with Adipose-Derived Stem Cells Stem Cells Translational Medicine 2022-07-20 [PMID: 35579982]

Bioinformatics

• Gene Symbol: MRC1
• Uniprot:
  • Q61830()