Monocyte/Granulocyte Antibody (74-22-15) [FITC]

Flow Cytometry: Monocyte/Granulocyte Antibody (74-22-15) [FITC] [NBP1-28242] - Porcine peripheral blood lymphocytes, monocytes, and granulocytes were stained with Mouse Anti-Porcine Monocyte/Granulocyte-FITC and Mouse Anti-Porcine CD3 -epsilon- PE.

Flow Cytometry: Monocyte/Granulocyte Antibody (74-22-15) [FITC] [NBP1-28242] - 1 ug/10^6 cells Fresh heparinized blood from a healthy adult pig was stained with mouse anti-pig Monocyte/Granulocyte-FITC. Red blood cells were lyzed with ammonium chloride and the cells were then fixed in 1% paraformaldehyde. Granulocytes were gated and analyzed on a flow cytometer

Flow Cytometry: Monocyte/Granulocyte Antibody (74-22-15) [FITC] [NBP1-28242] - Porcine peripheral blood granulocytes were stained with Mouse Anti-Porcine Monocyte/Granulocyte-PE (NBP1-28244).

• Reactivity: Mu, Po
• Applications: ELISA, ICC/IF
• Clone: 74-22-15
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC

Monocyte/Granulocyte Antibody (74-22-15) [FITC] Summary

• Immunogen: Fresh dd miniature swine thymocytes
• Specificity: Porcine SWC3a
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Purity: Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for Monocyte/Granulocyte Antibody (74-22-15) [FITC]

• 3-10C
• AMCF-I
• C-X-C motif chemokine 8
• CXCL8SCYB8
• Emoctakin
• GCP-1TSG-1
• interleukin 8
• K60
• LECT
• MDNCFb-ENAP
• member 8
• MONAPGCP1
• NAP-1NAP1
• Neutrophil-activating protein 1
• Protein 3-10C
• T cell chemotactic factor
• T-cell chemotactic factor

Background

MAb 74-22-15 reacts with >90% of pig monocytes and granulocytes and <5% of peripheral blood lymphocytes. This clone was clustered as anti-SWC3a at the First International Swine CD Workshop. (1-2)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.