Monocyte/Macrophage Antibody (KUL01) - Azide and BSA Free

Flow Cytometry: Monocyte/Macrophage Antibody (KUL01) [NBP1-28359] - Analysis using the FITC conjugate of NBP1-28359. Multiple staining of peripheral blood mononuclear cells.

• Reactivity: Ch
• Applications: Flow
• Clone: KUL01
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

Monocyte/Macrophage Antibody (KUL01) - Azide and BSA Free Summary

• Immunogen: Chicken peripheral blood mononuclear cell leukocytes
• Specificity: Chicken monocytes and macrophages
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry <= 1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: BBS (pH 8.2)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for Monocyte/Macrophage Antibody (KUL01) - Azide and BSA Free

• macrophage
• macrophages
• monocyte
• monocytes

Background

The monoclonal antibody KUL01 is useful in the study of the development, distribution, function and ontogeny of the mononuclear phagocyte system (MPS) of the chicken by exclusively recognizing the cells of the MPS. It identifies chicken monocytes and macrophages as well as interdigitating cells and activated microglia cells. This antibody does not react with B (Bu-1+) or T (CD3+) lymphocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Monocyte/Macrophage Antibody (NBP1-28359). (Showing 1 - 1 of 1 FAQ).

1. Is there a protocol available for the use of this antibody in parrafin embedded chicken tissue slides?
   • Unfortunately, we did not use any customized protocol for validating this product, however, please refer to our general IHC protocol. We recommend a dilution range of 1:10-1:2000 of the primary antibody for IHC. Moreover, we would encourage you to follow the research publication by the inventor of this antibody - Mast et al. 1998 (Vet Immunol Immunopathol. 61(2-4):343-57), wherein this product was validated for FLOW and IHC applications.