Monomethyl Arginine Antibody (5D1) - Azide and BSA Free

• Reactivity: All
• Applications: WB, ELISA, IHC
• Clone: 5D1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Monomethyl Arginine Antibody (5D1) - Azide and BSA Free Summary

• Immunogen: Modified monomethyl arginine
• Specificity: This reacts with free and bound NG-monomethyl arginine. There is no cross reaction with either NG-NG-dimethyl arginine or arginine.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:100 - 1:10 000
  • Immunohistochemistry-Paraffin 1:100-1:10000
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris , 0.1M Glycine and 2% Sucrose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Lyophilized powder, reconstituted in distilled water.

Alternate Names for Monomethyl Arginine Antibody (5D1) - Azide and BSA Free

• Arginine (mono methyl)
• Di methyl Arginine
• Dimethyl Arginine
• Methyl Arginine
• Mono methyl Arginine
• Monomethyl Arginine

Background

This antibody will be of central importance in analysing the methylation status of chromatin and transcription factors. Since it recognizes monomethyl arginine only it can be used in parallel with ab413 (detects dimethyl arginine only) and ab412 (detects both monomethyl and dimethyl arginine) to monitor the exact modification status.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Monomethyl Arginine Antibody (NB500-122)(2)

Publications using NB500-122

• Roberts JL, Liu G, Paglia DN et al. Deletion of Wnt5a in osteoclasts results in bone loss through decreased bone formation Annals of the New York Academy of Sciences 2020-03-01 [PMID: 31919867]
• Cuthbert GL, Daujat S, Snowden AW et al. Histone deimination antagonizes arginine methylation. Cell. 2004-09-01 [PMID: 15339660]