Mouse CD3+ T Cell Isolation Kit

Flow Cytometry: Mouse CD3+ T Cell Isolation Kit [MAGM201-NOV] - Mouse splenocytes before (A) and after (B) isolation of CD3+ T cells using the MagCellect Mouse CD3+ T Cell Isolation Kit. Histograms reflect all viable cells stained with CD3-FITC. Purity of isolated cells for this experiment was 97.5%

Product Discontinued

Mouse CD3+ T Cell Isolation Kit Summary

• Description: The MagCellect Mouse CD3+ T Cell Isolation Kit is designed to isolate CD3+ T cells via a negative selection principle. The resulting cell preparation is highly enriched with CD3+ T cells. Typical recovery ranges from 45-65% and the purity of recovered CD3+ T cells ranges from 90-98%.
• Kit Type: Isolation Kit
• Gene: CD3E

Applications/Dilutions

• Dilutions:
  • Flow Cytometry

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Background

The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction. MagCellect technology is based on the use of ferrofluids or magnetic nanoparticles that have no magnetic memory (superparamagnetic) and behave like colloidal particles. This feature allows the ferrofluids to remain in solution without the need for mixing and additionally allows for efficient diffusion kinetics during the binding reaction. The proprietary manufacturing technology of MagCellect(TM) Ferrofluids generates particles with higher ligand binding capacity per mass compared to many other larger diameter magnetic particles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CD3E