Mouse CD4+CD25+ Regulatory T Cell Isolation Kit

Flow Cytometry: Mouse CD4+CD25+ Regulatory T Cell Isolation Kit [MAGM208-NOV] - Mouse splenocytes were prepared as described in the Cell Preparation Section. Cells were stained with Rat Anti-Mouse CD4-FITC Monoclonal Antibody and Rat Anti-Mouse CD25-PE Monoclonal Antibody either before (Pre) or after (Post) isolation of CD4+CD25+ cells.

Flow Cytometry: Mouse CD4+CD25+ Regulatory T Cell Isolation Kit [MAGM208-NOV] - In the first step, CD4+ T cells were isolated from splenocytes. In the second step, CD4+CD25+ T cells were isolated from the CD4+ cells recovered in Step 1. CD4+CD25+ T cells were stained using FITC-conjugated Rat Anti-Mouse CD4 Monoclonal Antibody (Catalog # FAB554F) and PE-conjugated Rat Anti-Mouse CD25 Monoclonal Antibody (Catalog # FAB2438P).

Product Discontinued

Mouse CD4+CD25+ Regulatory T Cell Isolation Kit Summary

• Description: The MagCellect Mouse CD4+CD25+ Regulatory T Cell Isolation Kit is designed to isolate mouse CD4+CD25+ regulatory T cells from a preparation of splenocytes using a two-step procedure that combines both negative and positive selection techniques. The resulting cell preparation is highly enriched with CD4+CD25+ T cells with a purity of recovered cells >60%.
  
  Features
  • Yields highly enriched populations (84-94%) of Treg cells
  • Does not require specialized instrumentation
  • Obtains isolated Treg cells by a combination of negative and positive selection
• Kit Type: Isolation Kit

Applications/Dilutions

• Dilutions:
  • Flow Cytometry

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Background

Regulatory T cells (Treg), are a minor subset of CD4+ T cells that can suppress the development and progression of immune reactions. They also play an important role in the balance between tolerance and autoimmunity. Treg cells are characterized by the expression of CD4 and CD25 on the cell surface and the intracellular transcription factor FoxP3. The isolation of regulatory T cells facilitates research into their critical role in immune system regulation. MagCellect technology is based on the use of ferrofluids or magnetic nanoparticles that have no magnetic memory (superparamagnetic) and behave like colloidal particles. This feature allows the ferrofluids to remain in solution without the need for mixing and additionally allows for efficient diffusion kinetics during the binding reaction. The proprietary manufacturing technology of MagCellect(TM) Ferrofluids generates particles with higher ligand binding capacity per mass compared to many other larger diameter magnetic particles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.