Mouse CD4+ T Cell Isolation Kit

Flow Cytometry: Mouse CD4+ T Cell Isolation Kit [MAGM202-NOV] - Ficolled mouse splenocytes before (A) and after (B) isolation of CD4+ T cells were stained with PE-conjugated Rat Anti-Mouse CD4 Monoclonal Antibody and FITC-conjugated Rat Anti-Mouse CD3 Monoclonal Antibody.

Product Discontinued

Mouse CD4+ T Cell Isolation Kit Summary

• Description: The MagCellect Mouse CD4+ T Cell Isolation Kit is designed to isolate CD4+ T cells via a negative selection principle. The resulting cell preparation is highly enriched with CD4+ T cells. Typical recovery ranges between 40% and 66% and the purity of recovered CD4+ T cells ranges from 90-95%, with no detectable CD8+ T cells.
  
  Features
  • Yields highly pure (90-95%) CD4+ T cells in under an hour
  • Isolated CD4+ T cells are untouched by beads or antibodies
  • Cost effective for the processing of multiple samples in combination with Magnet (Catalog # MAG997-NOV)
  • Negative selection reduces sources of experimental variation
• Kit Type: Isolation Kit
• Gene: CD4

Applications/Dilutions

• Dilutions:
  • Flow Cytometry

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Background

CD4+ T cells develop in the thymus and differentiate into various subsets of more specialized T lymphocytes. The development of these subsets is induced by distinct signals and is controlled by distinct transcription factors. CD4+ T cell subsets produce characteristic combinations of cytokines which enables them to exert diverse functions. CD4+ T cells are critically involved in recruiting and activating other immune cells, dampening ongoing immune responses, and maintaining immunologic memory. The relative expansion of CD4+ subsets changes dramatically in response to local environmental stresses or disease status. The isolation of CD4+ T cells is an important step in the preparation of multiple subsets including Th1, Th2, and Th17 cells. MagCellect technology is based on the use of ferrofluids or magnetic nanoparticles that have no magnetic memory (superparamagnetic) and behave like colloidal particles. This feature allows the ferrofluids to remain in solution without the need for mixing and additionally allows for efficient diffusion kinetics during the binding reaction. The proprietary manufacturing technology of MagCellect(TM) Ferrofluids generates particles with higher ligand binding capacity per mass compared to many other larger diameter magnetic particles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CD4