Mouse Hematopoietic Non-Committed Lineage Cell Isolation Kit

Flow Cytometry: Mouse Hematopoietic Non-Committed Lineage Cell Isolation Kit [MAGM209-NOV] - CD117 (c-kit) staining of bone marrow lineage negative cells isolated from BALB/c mice using this kit. Histograms reflect reactivity of all viable cells with CD117-PE or a matched isotype control. Inset shows CD117 staining of cells before isolation.

Flow Cytometry: Mouse Hematopoietic Non-Committed Lineage Cell Isolation Kit [MAGM209-NOV] - SCF R/c-kit staining of bone marrow lineage-negative cells isolated from BALB/c mice using the MagCellect Mouse Hematopoietic Cell Lineage Depletion Kit. Histograms reflect the reactivity of all viable cells with a PE-conjugated Rat Anti-Mouse SCF R/c-kit Monoclonal Antibody (Filled histogram) or a PE-conjugated Rat IgG2A Isotype Control (open histogram) before (A) or (B) after lineage depletion.

Flow Cytometry: Mouse Hematopoietic Non-Committed Lineage Cell Isolation Kit [MAGM209-NOV] - Lineage marker reactivity on BALB/c bone marrow (BM) cells processed with the MagCellect Mouse Hematopoietic Cell Lineage Depletion Kit. The histograms show the reactivity of BM cells labeled with the cocktail of biotinylated antibodies (Rat Anti-Mouse CD5, CD11b, B220/CD45R, Gr-1/Ly-6G, and TER-119) included in the kit both before (green histogram) and after (purple histogram) magnetic depletion. Lineage marker reactivity was detected using Streptavidin-PE.

Flow Cytometry: Mouse Hematopoietic Non-Committed Lineage Cell Isolation Kit [MAGM209-NOV] - Lineage marker reactivity on BALB/c bone marrow (BM) cells processed with this kit. Histograms show reactivity of BM cells labeled with the cocktail of biotinylated antibodies before (filled) and after (open) magentic depletion. Lineage marker reactivity was developed by using Streptavidin-PE.

Product Discontinued

Mouse Hematopoietic Non-Committed Lineage Cell Isolation Kit Summary

• Description: The MagCellect Mouse Hematopoietic Cell Lineage Depletion Kit is designed for the isolation of hematopoietic non lineage committed cells (lineage negative) from mouse bone marrow. Lineage committed cells (lineage positive) targeted for magnetic depletion include T cells, B cells, NK cells, monocytes/macrophages, granulocytes, and erythrocytes. The resulting cell population is highly enriched for CD117+ cells (from 40-70% depending on mouse strain) with less than 5% residual lineage positive cells.
  
  Features
  • Less than 5% residual lineage-positive cells remain after depletion
  • Isolated HSCs are untouched by beads or antibodies
  • Negative selection reduces sources of experimental variation
• Kit Type: Isolation Kit

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Background

The definitive hematopoietic system is made up of all adult blood cell types including megakaryocytes, erythrocytes, and cells of the myeloid and lymphoid lineages. All of these cells are derived from multipotent hematopoietic stem cells (HSCs) through a succession of precursors with progressively limited potential. Hematopoietic stem cells are tissue-specific stem cells that exhibit remarkable self-renewal capacity and are responsible for the life-long maintenance of the hematopoietic system. HSCs are rare cells that reside in adult bone marrow where hematopoiesis is continuously taking place. They can also be found in cord blood, fetal liver, adult spleen, and peripheral blood. R&D Systems offers several products for studying hematopoietic lineage cells including serum-free media, lineage depletion antibodies and kits, and reagents for performing colony forming cell (CFC) assays. MagCellect technology is based on the use of ferrofluids or magnetic nanoparticles that have no magnetic memory (superparamagnetic) and behave like colloidal particles. This feature allows the ferrofluids to remain in solution without the need for mixing and additionally allows for efficient diffusion kinetics during the binding reaction. The proprietary manufacturing technology of MagCellect(TM) Ferrofluids generates particles with higher ligand binding capacity per mass compared to many other larger diameter magnetic particles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.