Mouse Naive CD4+ T Cell Isolation Kit

Flow Cytometry: Mouse Naive CD4+ T Cell Isolation Kit [MAGM205-NOV] - Mouse splenocytes before (A) and after (B) isolation of Naive CD4+ T cells using the MagCellect . Dot plots reflect double staining of all viable cells with CD44-FITC/CD62L-PE. Of all isolated cells (B) 97% were CD3+/CD4+.

Product Discontinued

Mouse Naive CD4+ T Cell Isolation Kit Summary

• Description: The MagCellect Mouse Naive CD4+ T Cell Isolation Kit is designed to isolate naive CD4+ T cells via a negative selection principle. The resulting cell preparation is highly enriched with naive CD4+ T cells (CD3+/CD4+/CD62L+/CD44low). Typical recoveries range from 5-35% and the purity of recovered cells ranges from 85-95%
  
  Features
  • Yields a highly pure (85-95%) population of mouse naive CD4+ T cells
  • Isolated naive CD4+ T cells are untouched by beads or antibodies
  • Cost effective for the processing of multiple samples in combination with Magnet (Catalog #MAG997-NOV)
  • Negative selection reduces sources of experimental variation
• Kit Type: Isolation Kit
• Gene: CD4

Applications/Dilutions

• Dilutions:
  • Flow Cytometry

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Background

Mouse CD4+ T cells develop in the thymus and are released as naive T cells that also express CD62L and low levels of CD44. These cells differentiate into subsets of specialized effector T lymphocytes in response to distinct environmental cues and the activation of specific transcription factors. The isolation of CD4+ T cells is an important step in the preparation of multiple subsets including Th1, Th2, Th17, Th9, Th22, and regulatory T cells. CD4+ T cell subsets secrete characteristic combinations of cytokines which enables them to exert diverse functions including the recruitment and activation of additional immune cells, the dampening of ongoing immune responses, and the maintenance of immunologic memory. MagCellect technology is based on the use of ferrofluids or magnetic nanoparticles that have no magnetic memory (superparamagnetic) and behave like colloidal particles. This feature allows the ferrofluids to remain in solution without the need for mixing and additionally allows for efficient diffusion kinetics during the binding reaction. The proprietary manufacturing technology of MagCellect(TM) Ferrofluids generates particles with higher ligand binding capacity per mass compared to many other larger diameter magnetic particles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CD4