Mouse NK Cell Isolation Kit

Flow Cytometry: Mouse NK Cell Isolation Kit [MAGM210-NOV] - Cells before and (B) after enrichment were double-stained with APC-conjugated anti-mouse CD49b and PE-conjugated anti-mouse NKp46 to specifically label the NK cell population. (C) A small population with dim staining for CD49b and NKp46 is also enriched. Before selection (filled histogram), after selection (open histogram).

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Mouse NK Cell Isolation Kit Summary

• Description: The MagCellect Mouse NK Cell Isolation Kit is designed to isolate natural killer cells via a negative selection principle. The resulting cell preparation is highly enriched with NK cells. The purity of recovered cells ranges from 70-80%.
• Kit Type: Isolation Kit

Applications/Dilutions

• Dilutions:
  • Flow Cytometry

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Background

Several natural killer (NK) cell subsets have been described in humans and mice based on their phenotypic markers and functional capabilities. Human natural killer (NK) cells have been defined based on the level of cell surface expression of NCAM-1/CD56 and CD16 on CD3- lymphocytes. CD56dimCD16+ cells are found predominantly in the blood and spleen and are highly cytotoxic, while CD56brightCD16- NK cells are found in the lymph nodes and tonsils. CD56brightCD16- cells are potent cytokine producers, but have only weak cytotoxic potential prior to activation. In mouse, natural killer cells are defined based on the expression of CD161/NK1.1, Integrin alpha 2/CD49b, NKp46, Integrin alpha M/CD11b and CD27 on CD3- lymphocytes. Within this population of cells, three different subsets of mouse NK cells have been identified: CD11dimCD27bright, CD11bbrightCD27dim, and CD11bbrightCD27bright NK cells. These three subsets differ in their tissue distribution, cytokine production, and cytotoxicity. CD11bdimCD27bright cells are found primarily in the bone marrow and lymph nodes, CD11bbrightCD27dim cells are localized predominantly in the blood, spleen, lung, and liver, and CD11bbrightCD27bright cells are widely distributed. Upon stimulation, CD27bright NK cells produce higher levels of cytokines and are more cytotoxic than CD27dim cells. MagCellect technology is based on the use of ferrofluids or magnetic nanoparticles that have no magnetic memory (superparamagnetic) and behave like colloidal particles. This feature allows the ferrofluids to remain in solution without the need for mixing and additionally allows for efficient diffusion kinetics during the binding reaction. The proprietary manufacturing technology of MagCellect(TM) Ferrofluids generates particles with higher ligand binding capacity per mass compared to many other larger diameter magnetic particles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.