Mouse/Rat GDF-15 Quantikine ELISA Kit

• Reactivity: Mu, Rt
• Applications: ELISA
• Conjugate: HRP

Mouse/Rat GDF-15 Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse/Rat GDF-15 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse and rat GDF-15 in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant mouse GDF-15 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using ...natural mouse or rat GDF-15 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse and rat GDF-15.
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• Specificity: Natural mouse and rat GDF-15, recombinant mouse mature GDF-15, precursor GDF-15, and GDF-15/BMP-2 heterodimer. It does not detect monomeric recombinant mouse GDF-15.
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Gdf15

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse/Rat GDF-15 Quantikine ELISA Kit

• GDF15
• GDF-15
• growth differentiation factor 15
• growth/differentiation factor 15
• Macrophage inhibitory cytokine 1
• MIC-1
• MIC-1NSAID-activated gene 1 protein
• MIC1Prostate differentiation factor
• NAG-1
• NAG-1NSAID-regulated gene 1 protein
• NSAID (nonsteroidal inflammatory drug)-activated protein 1
• PDF
• PDFGDF-15
• PLAB
• PLABNRG-1
• Placental bone morphogenetic protein
• Placental TGF-beta
• PTGF-beta
• PTGFBPTGF-beta

Background

Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for GDF-15 (MGD150)(42)

Publications using MGD150

• Fu, W;Lai, Y;Li, K;Yang, Y;Guo, X;Gong, Q;Zhou, X;Zhou, L;Liu, C;Zhang, Z;So, J;Zhang, Y;Huang, L;Lu, G;Yi, C;Wang, Q;Fan, C;Liu, C;Wang, J;Yu, H;Zhao, Y;Huang, T;Roh, HC;Liu, T;Tang, H;Qi, J;Xu, M;Zheng, Y;Huang, H;Li, J; Neurotensin-neurotensin receptor 2 signaling in adipocytes suppresses food intake through regulating ceramide metabolism Cell research 2025-01-03 [PMID: 39748047] (Mouse)
• Yulyaningsih, E;Suh, JH;Fanok, M;Chau, R;Solanoy, H;Takahashi, R;Bakardjiev, AI;Becerra, I;Benitez, NB;Chiu, CL;Davis, SS;Dowdle, WE;Earr, T;Estrada, AA;Gill, A;Ha, C;Haddick, PCG;Henne, KR;Larhammar, M;Leung, AW;Maciuca, R;Memarzadeh, B;Nguyen, HN;Nugent, AA;Osipov, M;Ran, Y;Rebadulla, K;Roche, E;Sandman, T;Wang, J;Lewcock, JW;Scearce-Levie, K;Kane, LA;Sanchez, PE; DNL343 is an investigational CNS penetrant eukaryotic initiation factor 2B activator that prevents and reverses the effects of neurodegeneration caused by the integrated stress response eLife 2024-09-17 [PMID: 39287504] (Mouse)
• Labour, A;Lac, M;Frassin, L;Lair, B;Murphy, E;Maslo, C;Monbrun, L;Calmy, ML;Marquès, M;Viguerie, N;Tavernier, G;Gourdy, P;O'Gorman, D;Montastier, E;Laurens, C;Montagner, A;Moro, C; GDF15 is dispensable for the insulin-sensitizing effects of chronic exercise Cell reports 2024-08-02 [PMID: 39096490] (Mouse)
• Mulcahy, MC;El Habbal, N;Redd, JR;Sun, H;Gregg, BE;Bridges, D; GDF15 knockout does not substantially impact perinatal body weight or neonatal outcomes in mice bioRxiv : the preprint server for biology 2024-05-03 [PMID: 38746399] (Mouse)
• Lund, C;Ranea-Robles, P;Falk, S;Rausch, DM;Skovbjerg, G;Vibe-Petersen, VK;Krauth, N;Skytte, JL;Vana, V;Roostalu, U;Pers, TH;Lund, J;Clemmensen, C; Protection against overfeeding-induced weight gain is preserved in obesity but does not require FGF21 or MC4R Nature communications 2024-02-08 [PMID: 38331907] (Mouse)
• Guo, X;Asthana, P;Zhai, L;Cheng, KW;Gurung, S;Huang, J;Wu, J;Zhang, Y;Mahato, AK;Saarma, M;Ustav, M;Kwan, HY;Lyu, A;Chan, KM;Xu, P;Bian, ZX;Wong, HLX; Artesunate treats obesity in male mice and non-human primates through GDF15/GFRAL signalling axis Nature communications 2024-02-03 [PMID: 38310105] (Mouse)
• Jena, J;García-Peña, LM;Weatherford, ET;Marti, A;Bjorkman, SH;Kato, K;Koneru, J;Chen, JH;Seeley, RJ;Abel, ED;Pereira, RO; GDF15 is required for cold-induced thermogenesis and contributes to improved systemic metabolic health following loss of OPA1 in brown adipocytes eLife 2023-10-11 [PMID: 37819027] (Mouse)
• Kojima, Y;Mishiro-Sato, E;Fujishita, T;Satoh, K;Kajino-Sakamoto, R;Oze, I;Nozawa, K;Narita, Y;Ogata, T;Matsuo, K;Muro, K;Taketo, MM;Soga, T;Aoki, M; Decreased liver B vitamin-related enzymes as a metabolic hallmark of cancer cachexia Nature communications 2023-10-06 [PMID: 37803016] (Transgenic Mouse, Mouse, Xenograft)
• Tezze, C;Amendolagine, FI;Nogara, L;Baraldo, M;Ciciliot, S;Arcidiacono, D;Zaramella, A;Masiero, G;Ferrarese, G;Realdon, S;Blaauw, B;Detienne, G;Beliën, AT;Sandri, M;Mercken, EM; A combination of metformin and galantamine exhibits synergistic benefits in the treatment of sarcopenia JCI insight 2023-08-08 [PMID: 37551712] (Mouse)
• Kanta, JM;Deisen, L;Johann, K;Holm, S;Lundsgaard, A;Lund, J;Jähnert, M;Schürmann, A;Clemmensen, C;Kiens, B;Fritzen, AM;Kleinert, M; Dietary medium-chain fatty acids reduce food intake via the GDF15-GFRAL axis in mice Molecular metabolism 2023-06-24 [PMID: 37356805] (Mouse)
• Klein Hazebroek, M;Laterveer, R;Kutschke, M;Ramšak Mar?eta, V;Barthem, CS;Keipert, S; Hyperphagia of female UCP1-deficient mice blunts anti-obesity effects of FGF21 Scientific reports 2023-06-24 [PMID: 37355753] (Mouse)
• Singh, A;Rourk, K;Bernier, A;de Lartigue, G; Non-Nutritive Sweetened Beverages Impair Therapeutic Benefits of Metformin in Prediabetic Diet-Induced Obese Mice Nutrients 2023-05-25 [PMID: 37299435] (Mouse)
• Southwell, N;Primiano, G;Nadkarni, V;Attarwala, N;Beattie, E;Miller, D;Alam, S;Liparulo, I;Shurubor, YI;Valentino, ML;Carelli, V;Servidei, S;Gross, SS;Manfredi, G;Chen, Q;D'Aurelio, M; A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy EMBO molecular medicine 2023-05-24 [PMID: 37222423] (Mouse)
• JY Kim-Muller, L Song, B LaCarubba, E Pashos, X Li, A Rinaldi, S Joaquim, JC Stansfield, J Zhang, A Robertson, J Pang, A Opsahl, M Boucher, D Breen, K Hales, A Sheikh, Z Wu, BB Zhang GDF15 neutralization restores muscle function and physical performance in a mouse model of cancer cachexia Cell Reports, 2023-01-10;42(1):111947. 2023-01-10 [PMID: 36640326] (Mouse)
• RC Zapata, M Carretero, FCG Reis, BS Chaudry, J Ofrecio, D Zhang, R Sasik, T Ciaraldi, M Petraschec, O Osborn Adipocytes control food intake and weight regain via Vacuolar-type H+ ATPase Nature Communications, 2022-08-30;13(1):5092. 2022-08-30 [PMID: 36042358] (Mouse)
• Y Ni, GH Wu, JJ Cai, R Zhang, Y Zheng, JQ Liu, XH Yang, X Yang, Y Shen, JM Lai, XM Ye, SJ Mo Tubule-mitophagic secretion of SerpinG1 reprograms macrophages to instruct anti-septic acute kidney injury efficacy of high-dose ascorbate mediated by NRF2 transactivation International journal of biological sciences, 2022-08-08;18(13):5168-5184. 2022-08-08 [PMID: 35982894] (Mouse)
• AL Queiroz, E Dantas, S Ramsamooj, A Murthy, M Ahmed, ERM Zunica, RJ Liang, J Murphy, CD Holman, CJ Bare, G Ghahramani, Z Wu, DE Cohen, JP Kirwan, LC Cantley, CL Axelrod, MD Goncalves Blocking ActRIIB and restoring appetite reverses cachexia and improves survival in mice with lung cancer Nature Communications, 2022-08-08;13(1):4633. 2022-08-08 [PMID: 35941104] (Human)
• S Maschalidi, P Mehrotra, BN Keçeli, HKL De Cleene, K Lecomte, R Van der Cr, P Janssen, J Pinney, G van Loo, D Elewaut, A Massie, E Hoste, KS Ravichandr Targeting SLC7A11 improves efferocytosis by dendritic cells and wound healing in diabetes Nature, 2022-05-25;0(0):. 2022-05-25 [PMID: 35614212] (Mouse)
• GS McElroy, RP Chakrabart, KB D'Alessand, K Vasan, J Tan, JS Stoolman, SE Weinberg, EM Steinert, PA Reyfman, BD Singer, WC Ladiges, L Gao, J Lopéz-Barn, GRS Budinger, NS Chandel Reduced expression of mitochondrial complex I subunit Ndufs2 does not impact healthspan in mice Scientific Reports, 2022-03-25;12(1):5196. 2022-03-25 [PMID: 35338200] (Mouse)
• L Morató, S Astori, I Zalachoras, J Rodrigues, S Ghosal, W Huang, I Guillot de, J Grosse, O Zanoletti, L Cao, J Auwerx, C Sandi eNAMPT actions through nucleus accumbens NAD+/SIRT1 link increased adiposity with sociability deficits programmed by peripuberty stress Science Advances, 2022-03-02;8(9):eabj9109. 2022-03-02 [PMID: 35235362] (Mouse)
• J Geppert, AA Walth, R Terrón Exp, D Kaltenecke, P Morigny, J Machado, M Becker, E Simoes, JDCC Lima, C Daniel, M Berriel Di, S Herzig, M Seelaender, M Rohm Aging Aggravates Cachexia in Tumor-Bearing Mice Cancers, 2021-12-24;14(1):. 2021-12-24 [PMID: 35008253] (Mouse)
• Y Moritoh, SI Abe, H Akiyama, A Kobayashi, R Koyama, R Hara, S Kasai, M Watanabe The enzymatic activity of inositol hexakisphosphate kinase controls circulating phosphate in mammals Nature Communications, 2021-08-11;12(1):4847. 2021-08-11 [PMID: 34381031] (Rat)
• M Yang, T Darwish, P Larraufie, D Rimmington, I Cimino, DA Goldspink, B Jenkins, A Koulman, CA Brighton, M Ma, BYH Lam, AP Coll, S O'Rahilly, F Reimann, FM Gribble Inhibition of mitochondrial function by metformin increases glucose uptake, glycolysis and GDF-15 release from intestinal cells Scientific Reports, 2021-01-28;11(1):2529. 2021-01-28 [PMID: 33510216] (Mouse)
• ES Selen, J Choi, MJ Wolfgang Discordant hepatic fatty acid oxidation and triglyceride hydrolysis leads to liver disease JCI Insight, 2021-01-25;6(2):. 2021-01-25 [PMID: 33491665] (Transgenic Mouse)
• AS Pereyra, A Rajan, CR Ferreira, JM Ellis Loss of Muscle Carnitine Palmitoyltransferase 2 Prevents Diet-Induced Obesity and Insulin Resistance despite Long-Chain Acylcarnitine Accumulation Cell Rep, 2020-11-10;33(6):108374. 2020-11-10 [PMID: 33176143] (Mouse)
• H Kim, KM Kim, MJ Kang, S Lim Growth differentiation factor-15 as a biomarker for sarcopenia in aging humans and mice Exp Gerontol, 2020-10-16;142(0):111115. 2020-10-16 [PMID: 33069782] (Mouse)
• CW Chian, YS Lee, YJ Lee, YH Chen, CP Wang, WC Lee, HJ Lee Cilostazol ameliorates diabetic nephropathy by inhibiting highglucose- induced apoptosis Korean J. Physiol. Pharmacol., 2020-09-01;24(5):403-412. 2020-09-01 [PMID: 32830147] (Rat)
• M Stolina, X Luo, D Dwyer, CY Han, R Chen, Y Zhang, Y Xiong, Y Chen, J Yin, A Shkumatov, B Ason, C Hale, MM Véniant The evolving systemic biomarker milieu in obese ZSF1 rat model of human cardiometabolic syndrome: Characterization of the model and cardioprotective effect of GDF15 PLoS ONE, 2020-08-17;15(8):e0231234. 2020-08-17 [PMID: 32804947] (Rat)
• ITN Nguyen, MM Brandt, J van de Wou, RWA van Drie, M Wesseling, MJ Cramer, SCA de Jager, D Merkus, DJ Duncker, C Cheng, JA Joles, MC Verhaar Both male and female obese ZSF1 rats develop cardiac dysfunction in obesity-induced heart failure with preserved ejection fraction PLoS ONE, 2020-05-06;15(5):e0232399. 2020-05-06 [PMID: 32374790] (Rat)
• RG Walker, O Barrandon, T Poggioli, S Dagdeviren, SH Carroll, MJ Mills, KR Mendello, Y Gomez, FS Loffredo, JR Pancoast, C Macias-Tre, C Marts, KB LeClair, HL Noh, T Kim, AS Banks, JK Kim, DE Cohen, AJ Wagers, DA Melton, RT Lee Exogenous GDF11, but not GDF8, reduces body weight and improves glucose homeostasis in mice Sci Rep, 2020-03-12;10(1):4561. 2020-03-12 [PMID: 32165710] (Mouse)
• CI Gil, M Ost, J Kasch, S Schumann, S Heider, S Klaus Role of GDF15 in active lifestyle induced metabolic adaptations and acute exercise response in mice Sci Rep, 2019-12-27;9(1):20120. 2019-12-27 [PMID: 31882966] (Mouse)
• D Flicker, Y Sancak, E Mick, O Goldberger, VK Mootha Exploring the In�Vivo Role of the Mitochondrial Calcium Uniporter in Brown Fat Bioenergetics Cell Rep, 2019-04-30;27(5):1364-1375.e5. 2019-04-30 [PMID: 31042465] (Mouse)
• T Tran, J Yang, J Gardner, Y Xiong GDF15 deficiency promotes high fat diet-induced obesity in mice PLoS ONE, 2018-08-02;13(8):e0201584. 2018-08-02 [PMID: 30070999] (Mouse)
• T Ito, Y Nakanishi, N Yamaji, S Murakami, SW Schaffer Induction of Growth Differentiation Factor 15 in Skeletal Muscle of Old Taurine Transporter Knockout Mouse Biol. Pharm. Bull., 2018-01-01;41(3):435-439. 2018-01-01 [PMID: 29491220] (Mouse)
• M Zhang, W Sun, J Qian, Y Tang Fasting exacerbates hepatic growth differentiation factor 15 to promote fatty acid ?-oxidation and ketogenesis via activating XBP1 signaling in liver Redox Biol, 2018-02-01;16(0):87-96. 2018-02-01 [PMID: 29482168] (Mouse)
• JE Jones, SM Cadena, C Gong, X Wang, Z Chen, SX Wang, C Vickers, H Chen, E Lach-Trifi, JR Hadcock, DJ Glass Supraphysiologic Administration of GDF11 Induces Cachexia in Part by Upregulating GDF15 Cell Rep, 2018-02-06;22(6):1522-1530. 2018-02-06 [PMID: 29425507] (Mouse)
• V Coleman, P Sa-Nguanmo, J Koenig, TJ Schulz, T Grune, S Klaus, AP Kipp, M Ost Partial involvement of Nrf2 in skeletal muscle mitohormesis as an adaptive response to mitochondrial uncoupling Sci Rep, 2018-02-05;8(1):2446. 2018-02-05 [PMID: 29402993] (Mouse)
• J Lee, J Choi, ES Selen Alpe, L Zhao, T Hartung, S Scafidi, RC Riddle, MJ Wolfgang Loss of Hepatic Mitochondrial Long-Chain Fatty Acid Oxidation Confers Resistance to Diet-Induced Obesity and Glucose Intolerance Cell Rep, 2017-07-18;20(3):655-667. 2017-07-18 [PMID: 28723568] (Mouse)
• T Matsuhashi, T Sato, SI Kanno, T Suzuki, A Matsuo, Y Oba, M Kikusato, E Ogasawara, T Kudo, K Suzuki, O Ohara, H Shimbo, F Nanto, H Yamaguchi, D Saigusa, Y Mukaiyama, A Watabe, K Kikuchi, H Shima, E Mishima, Y Akiyama, Y Oikawa, HO Hsin-Jung, Y Akiyama, C Suzuki, M Uematsu, M Ogata, N Kumagai, M Toyomizu, A Hozawa, N Mano, Y Owada, S Aiba, T Yanagisawa, Y Tomioka, S Kure, S Ito, K Nakada, KI Hayashi, H Osaka, T Abe Mitochonic Acid 5 (MA-5) Facilitates ATP Synthase Oligomerization and Cell Survival in Various Mitochondrial Diseases EBioMedicine, 2017-05-13;0(0):. 2017-05-13 [PMID: 28579242] (Mouse)
• FO Villars, C Pietra, C Giuliano, TA Lutz, T Riediger Oral Treatment with the Ghrelin Receptor Agonist HM01 Attenuates Cachexia in Mice Bearing Colon-26 (C26) Tumors Int J Mol Sci, 2017-05-05;18(5):. 2017-05-05 [PMID: 28475119] (Mouse)
• N Ban, CJ Siegfried, JB Lin, YB Shui, J Sein, W Pita-Thoma, A Sene, A Santeford, M Gordon, R Lamb, Z Dong, SC Kelly, V Cavalli, J Yoshino, RS Apte GDF15 is elevated in mice following retinal ganglion cell death and in glaucoma patients JCI Insight, 2017-05-04;2(9):. 2017-05-04 [PMID: 28469085] (Mouse, Rat)
• Fujimura K, Wakino S, Minakuchi H, Hasegawa K, Hosoya K, Komatsu M, Kaneko Y, Shinozuka K, Washida N, Kanda T, Tokuyama H, Hayashi K, Itoh H Ghrelin protects against renal damages induced by angiotensin-II via an antioxidative stress mechanism in mice. PLoS ONE, 2014-04-18;9(4):e94373. 2014-04-18 [PMID: 24747517] (Mouse)

Bioinformatics

• Gene Symbol: Gdf15