Mrc2 Antibody

Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Mrc2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5770) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Mrc2 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Mrc2 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human Mrc2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5770) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Mrc2 Antibody Summary

• Immunogen: E. coli-derived recombinant human Mrc2
  Cys800-Ala1059
  Accession # Q9UBG0
• Specificity: Detects human Mrc2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant mouse Mrc2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MRC2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mrc2 Antibody

• CD280 antigen
• CD280
• CD280C-type mannose receptor 2
• CLEC13E
• CLEC13EUPAR-associated protein
• Endo180
• ENDO180C-type lectin domain family 13 member E
• Endocytic receptor 180
• KIAA0709FLJ35911
• Macrophage mannose receptor 2
• mannose receptor, C type 2
• Mrc2
• uPARAP
• UPARAPendocytic receptor (macrophage mannose receptor family)
• urokinase plasminogen activator receptor-associated protein
• Urokinase receptor-associated protein
• Urokinase-type plasminogen activator receptor-associated protein

Background

Mrc2 (C-type Mannose Receptor 2; also MMR2, Endocytic Receptor 180 and uPARAP) is a 180‑200 kDa member of the macrophage mannose receptor family of proteins. It is widely expressed, being found on fibroblasts, monocytes/macrophages, endothelial cells, keratinocytes and myoepithelial cells. It binds the carbohydrates Man, Fuc and NAcGlc, type V collagen, and pro‑uPA. Mature human Mrc2 is a 1449 amino acid (aa) type I transmembrane protein. It contains a 1384 aa extracellular region and a 44 aa cytoplasmic domain. The extracellular region shows one ricin B‑type lectin domain (aa 41‑167), one fibronectin type II domain (aa 182‑230) and eight C‑type lectin domains (aa 244‑1393). Over aa 800‑1059, human Mrc2 shares 87% aa identity with mouse Mrc2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mrc2 Antibody (AF5770)(2)

Publications using AF5770

• Podolsky, MJ;Kheyfets, B;Pandey, M;Beigh, AH;Yang, CD;Lizama, CO;Datta, R;Lin, LL;Wang, Z;Wolters, PJ;McManus, MT;Qi, L;Atabai, K; Genome-wide screens identify SEL1L as an intracellular rheostat controlling collagen turnover Nature communications 2024-02-20 [PMID: 38378719] (Western Blot, Human)
• Michael J. Podolsky, Christopher D. Yang, Carlos Lizama Valenzuela, Ritwik Datta, Steven K. Huang, Stephen L. Nishimura et al. Age-dependent regulation of cell-mediated collagen turnover JCI Insight 2020-05-21 [PMID: 32315288]

Bioinformatics

• Gene Symbol: MRC2
• Uniprot:
  • Q9UBG0()