MTS Cell Proliferation Assay Kit (Colorimetric)

ELISA: MTS Cell Proliferation Assay Kit (Colorimetric) [NBP2-54884] - Human Insulin detected with Mouse anti Insulin/Proinsulin followed by HRP conjugated Rabbit F(ab')2 anti Mouse IgG.

ELISA: MTS Cell Proliferation Assay Kit (Colorimetric) [NBP2-54884] - Human Proinsulin detected with Mouse anti Insulin/Proinsulin antibody followed by HRP conjugated Rabbit F(ab')2 anti Mouse IgG antibody.

Product Discontinued

MTS Cell Proliferation Assay Kit (Colorimetric) Summary

• Description: Fast and sensitive MTS based assay to measure cell proliferation in response to growth factors, cytokines, mitogens and nutrients.
  
  Detection method: Absorbance at 490 - 500 nm.Applications: Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals. Assessment of physiological mediators that inhibit cell growth.
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab197010)
• Kit Type: Assay Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at -20C.

Background

The MTS Cell Proliferation Assay Kit is a colorimetric method for sensitive quantification of viable cells in proliferation and cytotoxicity assay. The method is based on the reduction of MTS tetrazolium compound by viable cells to generate a colored formazan product that is soluble in cell culture media. This conversion is thought to be carried out by NAD(P)H-dependent dehydrogenase enzymes in metabolically active cells. The formazan dye produced by viable cells can be quantified by measuring the absorbance at 490-500 nm. The assay can be used for the measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. It can also be used for the analysis of cytotoxic compounds like anticancer drugs and many other toxic agents and pharmaceutical compounds. The MTS assay is performed by adding the reagent directly into the cell culture media without the intermittent steps, which are required in the routine MTT assay. In addition, this high-throughput assay requires no washing or solubilization step and can be performed in 96-well microtiter plate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for MTS Cell Proliferation Kit (NBP2-54884)(1)

Publication using NBP2-54884

• Marashio A, Lambros M, Oyegbesan A Abstract 4047: Targeted liposomes against ovarian cancer Cancer Research 2022-06-15