MUC1 Antibody

Product Discontinued

MUC1 Antibody Summary

• Immunogen: Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) from cytoplasmic tail of human MUC-1.
• Localization: Cell Membrane and Cytoplasmic
• Specificity: MUC1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MUC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
• Application Notes: IHC-P: Use at a dilution of 1/100. Antigen retrieval is not essential but may optimise staining. Predicted molecular weight: 122 kDa. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 26002932)
• Theoretical MW: 350 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  MCF-7 Whole Cell Lysate
  MCF-7 Whole Cell Lysate

Reactivity Notes

Predicted by homology: Bovine, Rabbit, Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.6) and 1.0% BSA
• Preservative: 0.1% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MUC1 Antibody

• Breast carcinoma-associated antigen DF3
• Carcinoma-associated mucin
• CD227 antigen
• CD227
• DF3 antigen
• EMA
• Episialin
• H23 antigen
• H23AG
• KL-6
• MAM6
• MUC-1
• MUC1/ZD
• mucin 1, cell surface associated
• mucin 1, transmembrane
• Mucin-1
• Peanut-reactive urinary mucin
• PEM
• PEMMUC-1/SEC
• PEMT
• Polymorphic epithelial mucin
• PUMMUC-1/X
• tumor associated epithelial mucin
• Tumor-associated epithelial membrane antigen
• Tumor-associated mucin

Background

Mucin 1 (MUC1), also known as episialin, EMA (epithelial membrane antigen), PEM (polymorphic epithelial mucin), and CA-15-3 antigen, is a membrane-bound type I transmembrane glycoprotein (1,2). MUC1 is typically expressed in the luminal or glandular epithelial cells of the gastrointestinal tract, breast, lungs, and more, and is often overexpressed in epithelial cancers, but also serves a protective role against infection and helps regulate inflammatory response (2,3). Human MUC1 is 1255 amino acids (aa) in length with a theoretical molecular weight of 122 kDa; however, depending on the amount of glycosylation can weigh between 250 - 500 kDa (2,4). Structurally, MUC1 consists of a N-terminal domain which contains a signal peptide, a variable number tandem repeat region (VNTR), and a SEA domain, as well a C-terminal domain which has the extracellular domain (ECD), transmembrane domain (TMD), and cytoplasmic tail (CT) (2,3). The VNTR is comprised of between 25 - 125 repeats of a 20 aa conserved sequence (3). MUC1 is heavily O-glycosylated in the VNTR and has moderate N-glycosylation sites following the VNTR and in the ECD (2). Glycosylation contributes to MUC1's functional properties (2). The MUC1 gene contains seven exons, giving rise to several MUC1 isoforms as a result of alternative splicing (2).

Overexpression of mucins, including MUC1, is a feature of many epithelial cancers (1,3,5,6). The presence of truncated glycan structures called tumor-associated carbohydrate antigens (TACAs) on MUC1 play a role in cancer progression and a loss of apical-basal polarity (5). Carbohydrate-binding partners called lectins are the primary binding partners of TACAs that give rise to the pro-tumor microenvironment and metastasis (5). Given this unique feature, TACAs are a potential target for cancer immunotherapies (5). There are a number of vaccines, drugs, and antibodies targeting MUC1 for treatment of a variety of cancers including breast, lung, and prostate (6). In addition to a role in cancer progression, MUC1, and specifically the CT portion, has been shown to have a positive, anti-inflammatory role in a variety of lung and airway infections (7).

References

1. Khodabakhsh, F., Merikhian, P., Eisavand, M. R., & Farahmand, L. (2021). Crosstalk between MUC1 and VEGF in angiogenesis and metastasis: a review highlighting roles of the MUC1 with an emphasis on metastatic and angiogenic signaling. Cancer cell international. https://doi.org/10.1186/s12935-021-01899-8

2. Nath, S., & Mukherjee, P. (2014). MUC1: a multifaceted oncoprotein with a key role in cancer progression. Trends in molecular medicine. https://doi.org/10.1016/j.molmed.2014.02.007

3. Dhar, P., & McAuley, J. (2019). The Role of the Cell Surface Mucin MUC1 as a Barrier to Infection and Regulator of Inflammation. Frontiers in cellular and infection microbiology. https://doi.org/10.3389/fcimb.2019.00117

4. Uniprot (P15941)

5. Beckwith, D. M., & Cudic, M. (2020). Tumor-associated O-glycans of MUC1: Carriers of the glyco-code and targets for cancer vaccine design. Seminars in immunology. https://doi.org/10.1016/j.smim.2020.101389

6. Almasmoum H. (2021). The Roles of Transmembrane Mucins Located on Chromosome 7q22.1 in Colorectal Cancer. Cancer management and research. https://doi.org/10.2147/CMAR.S299089

7. Ballester, B., Milara, J., & Cortijo, J. (2021). The role of mucin 1 in respiratory diseases. European respiratory review : an official journal of the European Respiratory Society. https://doi.org/10.1183/16000617.0149-2020

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MUC1 Antibody (NB120-15481)(10)

Publications using NB120-15481

• Ezoe K, Murata N, Yabuuchi A et al. Evaluation of uterine receptivity after gonadotropin releasing hormone agonist administration as an oocyte maturation trigger: a rodent model Sci Rep 2019-08-29 [PMID: 31467307] (IF/IHC, Mouse)
• Fullerton PT, Monsivais D, Kommagani R, Matzuk MM. Follistatin is critical for mouse uterine receptivity and decidualization. Proc. Natl. Acad. Sci. U.S.A. 2017-06-13 [PMID: 28559342] (IHC-P, Mouse)
• Kelleher AM, Peng W, Pru JK et al. Forkhead box a2 (FOXA2) is essential for uterine function and fertility Proc. Natl. Acad. Sci. U.S.A 2017-01-03 [PMID: 28049832] (IHC-P, Mouse)
• Wang TS, Gao F, Qi QR et al. Dysregulated LIF-STAT3 pathway is responsible for impaired embryo implantation in a streptozotocin-induced diabetic mouse model. Biol Open. 2015-05-22 [PMID: 26002932] (ICC/IF, IHC-P, Mouse)
• Whiteman EL, Fan S, Harder JL et al. Crumbs3 is Essential for Proper Epithelial Development and Viability. Mol Cell Biol. 2013-10-28 [PMID: 24164893] (IF/IHC, ICC/IF, Mouse)
• Nallasamy S, Li Q, Bagchi MK, Bagchi IC. Msx Homeobox Genes Critically Regulate Embryo Implantation by Controlling Paracrine Signaling between Uterine Stroma and Epithelium. PLoS Genet;8(2):e1002500. 2012-02-01 [PMID: 22383889]
• Sekulovski N Tissue-Specific Ablation of Insulin Receptor Signaling Results in Infertility in Female Mice Thesis 2020-01-01 (Mouse)
• Kannan A, Davila J, Gao L Et al. Maternal high-fat diet during pregnancy with concurrent phthalate exposure leads to abnormal placentation Scientific reports 2021-08-16 [PMID: 34400704] (IHC-P, Mouse)
• Marinic M, Mika K, Chigurupati S, Lynch V Evolutionary transcriptomics implicates HAND2 in the origins of implantation and regulation of gestation length Elife 2021-02-01 [PMID: 33522483]
• Kelleher, A M, Burns, G W Et al. Uterine glands impact uterine receptivity, luminal fluid homeostasis and blastocyst implantation. Sci Rep 2016-12-01 [PMID: 27905495] (IF/IHC, Human)

Reviews for MUC1 Antibody (NB120-15481) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-P Mouse 05/05/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Species: Mouse
• Lot: 90900

reviewed by: Marion Pollheimer IHC-P Mouse 09/15/2010

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse Liver Tissue
• Species: Mouse

FAQs for MUC1 Antibody (NB120-15481). (Showing 1 - 2 of 2 FAQs).

1. I would like to perform a sandwich assay for the determination of MUC1 protein. May you suggest me some antibodies that can be used together? In particular, if possible, I should need antibodies product in mouse or rabbit.
   • Unfortunately we are not aware of a pair of antibodies that have been used in sandwich ELISA in particular. If you are interested in trying two you may find our Innovators Reward Program to be helpful.
2. I have a customer who is asking about whether MUC1 Partial Recombinant Protein from Novus (Cat# H00004582-Q01) can be used with MUC1 antibody (Cat# NB120-15481). Their question was  "Does the MUC1 antibody NB120-15481 have a high affinity for H00004582-Q01, given that it is recombinant. I need to find the protein with higher capacity for binding to that antibody." According to the product datasheet for the has been raised against a "Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) from cytoplasmic tail of human MUC-1". The partial recombinant MUC1 protein is made of "MUC1 (NP_877418.1, 315 a.a. - 420 a.a.)". So it depends if the MUC1 sequence that the MUC1 antibody was raised against is within this partial recombinant MUC1 protein sequence. The precise amino acid sequence of the immunogen for MUC1 antibody is not listed on the datasheet due to commercial sensitivity. Can you please advise if the amino acid sequence of the MUC1 antibody immunogen is within the partial recombinant MUC1 protein? If so, do you predict the antibody will bind this recombinant protein with high affinity? If not, is there an alternative product you can suggest? Just an additional note that the customer has just clarified: The products are to be used for "developing immunoassays with biosensors so essentially I need this antibody-protein pair for optimization of detection conditions before trying detection in real samples.
   • Our MUC1 antibody with catalogue number NB120-15481 was raised to a synthetic peptide derived from the cytoplasmic tail of human MUC1. According to the Uniprot listing (P15941) the cytoplasmic region of MUC1 is from amino acids 1182-1255. Our partial recombinant MUC1 protein with catalogue number H00004582-Q01 is derived from amino acids 315-420 so it is unlikely that NB120-15481 would recognise this protein. Unfortunately this partial recombinant protein is the only human MUC1 protein that we sell. This protein is produced by Abnova, for whom we distribute, but having checked their website I see that they do not stock any other MUC1 proteins either. Our MUC1 antibody with catalogue number NB300-708 was raised to a synthetic peptide representing a 20 amino acid repeat sequence from Mucin 1. A 60 amino acid long peptide was conjugated to KLH and was used to immunize rabbits. The 20 amino acid repeat sequence used was VTSAPDTRPAPGSTAPPAHG. This repeat sequence is present from amino acids 61-1020, and so it is entirely possible that NB300-708 will detect H00004582-Q01. We have not ourselves tested the binding of NB300-708 to the partial recombinant protein. But this may be a good option for your customer to try.

Bioinformatics

• Gene Symbol: MUC1
• Entrez:
  • 4582(Human)
  • 17829(Mouse)
• Uniprot:
  • P15941()