Murine Hepatitis Virus A59 nsp9 Antibody (2C6.H1) - BSA Free

Immunocytochemistry/Immunofluorescence: Murine Hepatitis Virus A59 nsp9 Antibody (2C6.H1) [NBP2-21671] - 6-h post infection in mouse L cells. Cells were fixed in 3% para-formaldehyde. For detection Cy2 conjugated Goat-anti-Mouse IgG MX10 was used.

Western blotting using a nsp9 antibody to detect protein in various lysates, 6h post MHV infection. Lane 1 shows no cross-reactivity with Sected Vero cells. Negative controls (lanes 2 and 4) show no staining. Specific reactivity against MHV-A59 nsp9 from infected mouse L cells is shown in lane 3. Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.

• Reactivity: V-Vi
• Applications: WB, DB, ICC/IF
• Clone: 2C6.H1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Murine Hepatitis Virus A59 nsp9 Antibody (2C6.H1) - BSA Free Summary

• Description: This product was purified from tissue culture supernatant fluid by Protein A chromatography
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This Murine Hepatitis Virus A59 nsp9 Antibody (2C6.H1) was produced in mice by repeated immunizations with E.coli derived full-length Murine Hepatitis Virus A59 nsp9 protein. This protein is part of the viral replicase polyprotein. (Uniprot: P0C6V0)
• Specificity: No cross reactivity occurs with SARS CoV nsp9.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ORF1ab
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Western Blot 1:1000
• Application Notes: This antibody has been tested for use in immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kDa in size corresponding to mature MHV-A59 nsp9 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells were grown on glass slides followed by infection with MHV-A59 strain and fixation with PBS/3% PFA. After washing and permeabilization of the fixed cells, antibody incubation was performed in PBS/5% FCS for 30 min.

Reactivity Notes

This antibody is directed against the Murine Hepatitis Virus A59 nsp9 protein. No cross reactivity occurs with SARS CoV nsp9. Cross reactivity with homologues from other sources has not been tested.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Alternate Names for Murine Hepatitis Virus A59 nsp9 Antibody (2C6.H1) - BSA Free

• 2'-O-MT
• 3' to 5' exonuclease
• 3C-like proteinase
• HEL1
• leader protein
• NendoU
• nsp10
• nsp11
• NSP2
• nsp4
• nsp6
• nsp7
• nsp8
• nsp9
• ORF1a polyprotein
• ORF1ab polyprotein
• PLpro
• polyprotein pp1a
• polyprotein pp1ab
• RNA-dependent RNA polymerase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Murine Hepatitis Virus A59 nsp9 Antibody (NBP2-21671)(1)

Publication using NBP2-21671

• Archer S, Dasgupta A, Chen K et al. SARS-CoV-2 mitochondriopathy in COVID-19 pneumonia exacerbates hypoxemia Redox Biology 2022-12-01 [PMID: 36334378] (IHC-P, Virus)
  
  Details:
  Dilution used in IHC 1:500

Bioinformatics

• Gene Symbol: ORF1ab
• Uniprot:
  • P0C6V0(Virus)