Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (2F11) - BSA Free

Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (2F11) [NBP2-22615] - The HepG2 cell lysate (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PFKM antibody (1:500 ~ 1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Flow Cytometry: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (2F11) [NBP2-22615] - Flow cytometry analysis of PFKM in Hep3B cell line, stainingat 2-5ug for 1x106cells (red line). The secondary antibodyused goat anti-mouse IgG Alexa fluor 488 conjugate. Isotypecontrol antibody was mouse IgG (black line).

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 2F11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (2F11) - BSA Free Summary

• Immunogen: Recombinant human Muscle Phosphofructokinase/PFKM/PFK-1(1-780aa) purified from E. coli
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PFKM
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (2F11) - BSA Free

• 6-phosphofructokinase, muscle type
• EC 2.7.1,6-phosphofructo-1-kinase
• EC 2.7.1.11
• GSD7
• MGC8699
• Muscle Phosphofructokinase
• PFK1
• PFK-1
• PFKA
• PFK-A
• PFKM
• PFKX
• Phosphofructo-1-kinase isozyme A
• Phosphofructokinase 1
• phosphofructokinase, muscle
• phosphofructokinase, polypeptide X
• Phosphofructokinase-1
• Phosphofructokinase-M
• Phosphohexokinase

Background

PFKM(phosphofructokinase, muscle) is a regulatory glycolytic enzyme that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PFKM
• Uniprot:
  • NP_000280(Human)