Recombinant Human Muscle Phosphofructokinase/PFKM/PFK-1 His Protein

SDS-Page: Recombinant Human Muscle Phosphofructokinase/PFKM/PFK-1 Protein [NBP1-44494] - PFKM, 87.3 kDa (800aa), confirmed by MALDI-TOF with a purity of 80% by SDS - PAGE

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human Muscle Phosphofructokinase/PFKM/PFK-1 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-780 of Human Muscle Phosphofructokinase/PFKM/PFK-1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MTHEEHHAAK TLGIGKAIAV LTSGGDAQGM NAAVRAVVRV GIFTGARVFF VHEGYQGLVD GGDHIKEATW ESVSMMLQLG GTVIGSARCK DFREREGRLR AAYNLVKRGI TNLCVIGGDG SLTGADTFRS EWSDLLSDLQ KAGKITDEEA TKSSYLNIVG LVGSIDNDFC GTDMTIGTDS ALHRIMEIVD AITTTAQSHQ RTFVLEVMGR HCGYLALVTS LSCGADWVFI PECPPDDDWE EHLCRRLSET RTRGSRLNII IVAEGAIDKN GKPITSEDIK NLVVKRLGYD TRVTVLGHVQ RGGTPSAFDR ILGSRMGVEA VMALLEGTPD TPACVVSLSG NQAVRLPLME CVQVTKDVTK AMDEKKFDEA LKLRGRSFMN NWEVYKLLAH VRPPVSKSGS HTVAVMNVGA PAAGMNAAVR STVRIGLIQG NRVLVVHDGF EGLAKGQIEE AGWSYVGGWT GQGGSKLGTK RTLPKKSFEQ ISANITKFNI QGLVIIGGFE AYTGGLELME GRKQFDELCI PFVVIPATVS NNVPGSDFSV GADTALNTIC TTCDRIKQSA AGTKRRVFII ETMGGYCGYL ATMAGLAAGA DAAYIFEEPF TIRDLQANVE HLVQKMKTTV KRGLVLRNEK CNENYTTDFI FNLYSEEGKG IFDSRKNVLG HMQQGGSPTP FDRNFATKMG AKAMNWMSGK IKESYRNGRI FANTPDSGCV LGMRKRALVF QPVAELKDQT DFEHRIPKEQ WWLKLRPILK ILAKYEIDLD TSDHAHLEHI TRKRSGEAAV

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PFKM
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 87.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 5mM DTT, 0.2 M NaCl, and 20% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Muscle Phosphofructokinase/PFKM/PFK-1 His Protein

• 6-phosphofructokinase, muscle type
• EC 2.7.1,6-phosphofructo-1-kinase
• EC 2.7.1.11
• GSD7
• MGC8699
• Muscle Phosphofructokinase
• PFK1
• PFK-1
• PFKA
• PFK-A
• PFKM
• PFKX
• Phosphofructo-1-kinase isozyme A
• Phosphofructokinase 1
• phosphofructokinase, muscle
• phosphofructokinase, polypeptide X
• Phosphofructokinase-1
• Phosphofructokinase-M
• Phosphohexokinase

Background

PFKM is regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Recombinant human PFKM protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Muscle Phosphofructokinase/PFKM/PFK-1 Recombinant Protein (NBP1-44494)(1)

Publication using NBP1-44494

• Park DI, Novak B, Yan Y et al. Paroxetine binding and activation of phosphofructokinase implicates energy metabolism in antidepressant mode of action J Psychiatr Res 2020-06-06 [PMID: 32540574]

FAQs for Muscle Phosphofructokinase/PFKM/PFK-1 Recombinant Protein (NBP1-44494). (Showing 1 - 1 of 1 FAQ).

1. Our company might be interested in Novus product PFK-1 Protein (NBP1-44494) (recombinant phosphofructokinase). Product page of Novus website does not indicate whether the protein is active or not. If the former, how this activity was tested? Could you please clarify on that?
   • This protein NBP1-44494 has not been tested for activity by the lab. As such we cannot guarantee if it is active or not and expect most likely it will not be active. Mainly this is intended as a control for Western Blot.

Bioinformatics

• Gene Symbol: PFKM