MxA/Mx1 Antibody (OTI2G12)

Western Blot: MxA/Mx1 Antibody (2G12) [NBP1-47859] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MX1.

Immunocytochemistry/Immunofluorescence: MxA/Mx1 Antibody (2G12) [NBP1-47859] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY MX1.

Western Blot: MxA/Mx1 Antibody (2G12) [NBP1-47859] - Analysis of extracts (10ug) from 1 cell lines by using anti- MX1 monoclonal antibody at 1:200 dilution

Western Blot: MxA/Mx1 Antibody (2G12) [NBP1-47859] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-MX1 monoclonal antibody.(1:200

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clone: OTI2G12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

MxA/Mx1 Antibody (OTI2G12) Summary

• Immunogen: Full length human recombinant protein of human MX1 (NP_002453) produced in HEK293T cell.
• Specificity: This antibody is specific for Homo sapiens myxovirus (influenza virus) resistance 1, interferon-inducible protein p78 (mouse) (MX1), transcript variant 2.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
• Theoretical MW: 75.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MxA/Mx1 Antibody (OTI2G12)

• human interferon-regulated resistance GTP-binding protein MXA10IFI-78Khomolog of murine (interferon-inducibleprotein p78)
• IFI78
• IFI-78K
• Mx1
• MxA
• myxovirus (influenza virus) resistance 1, interferon-inducible protein p78(mouse)

Background

In mouse, the interferon-inducible Mx protein is responsible for a specific antiviral state against influenza virus infection. The protein encoded by this gene is similar to the mouse protein as determined by its antigenic relatedness, induction conditions, physicochemical properties, and amino acid analysis. This cytoplasmic protein is a member of both the dynamin family and the family of large GTPases. Two transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MxA/Mx1 Antibody (NBP1-47859)(2)

Publications using NBP1-47859

• Shekarian T, Sivado E, Jallas AC et al. Repurposing rotavirus vaccines for intratumoral immunotherapy can overcome resistance to immune checkpoint blockade Sci Transl Med 2019-10-23 [PMID: 31645452]
• Frasca L, Palazzo R, Chimenti MS et al. Anti-LL37 Antibodies Are Present in Psoriatic Arthritis (PsA) Patients: New Biomarkers in PsA. Front Immunol. 2018-09-12 [PMID: 30279686] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: MX1
• Entrez:
  • 4599(Human)