Myeloid-Associated Differentiation Marker Antibody

Western Blot: Myeloid-Associated Differentiation Marker Antibody [NBP2-24494] - Total protein from mouse spleen, rat spleen and human stomach was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Myeloid Associated Protein in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.

Western Blot: Myeloid-Associated Differentiation Marker Antibody [NBP2-24494] - Total protein from mouse spleen, rat spleen and human stomach was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Myeloid Associated Protein in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.

Immunohistochemistry-Paraffin: Myeloid-Associated Differentiation Marker Antibody [NBP2-24494] - Immunohistochemical labeling was performed on formalin-fixed, paraffin embedded liver tissue microarrays. Data show 6 different sections (3 each from non-cancerous and cancer tissues). The MYADM antibody was used at 1:400 dilution. (Data courtesy of Dr. Akhilesh Pandey's laboratory at the Johns Hopkins University).

Flow Cytometry: Myeloid-Associated Differentiation Marker Antibody [NBP2-24494] - Flow analysis of AML cell line using Myeloid-Associated Differentiation Marker Antibody. Image from verified customer review.

Western Blot: Myeloid-Associated Differentiation Marker Antibody [NBP2-24494] - Human spleen lysate in the 1) absence and 2) presence of immunizing peptide and 3) rat spleen lysate probed with MYADM antibody at 0.25 ug/ml.

Immunohistochemistry-Paraffin: Myeloid-Associated Differentiation Marker Antibody [NBP2-24494] - Analysis of human tonsil using MYADM antibody at 10 ug/ml.

Product Discontinued

Myeloid-Associated Differentiation Marker Antibody Summary

• Immunogen: A portion of amino acids 250-300 of human MYADM was used as the immunogen for this antibody.
• Predicted Species: Mouse (90%), Orangutan (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MYADM
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5-10 ug/ml
  • Western Blot 0.2-1 ug/ml

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Myeloid-Associated Differentiation Marker Antibody

• MYADM
• Myeloid Upregulated Protein
• Protein SB135
• SB135

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myeloid-Associated Differentiation Marker Antibody (NBP2-24494)(2)

Publications using NBP2-24494

• Sun L, Lin P, Chen Y et al. miR-182-3p/Myadm contribute to pulmonary artery hypertension vascular remodeling via a KLF4/p21-dependent mechanism Theranostics 2020-04-25 [PMID: 32373233] (WB, ICC/IF)
• Chaerkady R, Harsha HC, Nalli A et al. A Quantitative Proteomic Approach for Identification of Potential Biomarkers in Hepatocellular Carcinoma. J Proteome Res 2008-10-01 [PMID: 18715028] (IHC-P)

Review for Myeloid-Associated Differentiation Marker Antibody (NBP2-24494) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 11/18/2015

Summary

• Application: Flow Cytometry
• Sample Tested: AML cell line
• Species: Human

Bioinformatics

• Gene Symbol: MYADM
• Uniprot:
  • Q96S97(Human)