Myeloperoxidase/MPO Activity Assay Kit (Colorimetric)

Myeloperoxidase/MPO Activity Assay Kit (Colorimetric) [NBP3-24523] - Assay Example

• Reactivity: All-NA
• Applications: Func
• Suitable Sample Type: Serum, plasma, milk, animal tissue and cell samples
• Standard Curve Range: 16.95 - 3349 U/L
• Sensitivity: 16.95 U/L

Myeloperoxidase/MPO Activity Assay Kit (Colorimetric) Summary

• Description: Detection instrument: Spectrophotometer (460 nm)
  Assay time: 90 min
• Standard Curve Range: 16.95 - 3349 U/L
• Sensitivity: 16.95 U/L
• Inter-Assay: %CV < 9.8
• Intra-Assay: %CV < 4.5
• Spike Recovery: Average recovery rate: 104%
• Kit Type: Activity Assay Kit (Colorimetric)
• Gene: MPO

Applications/Dilutions

• Dilutions:
  • Functional

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Myeloperoxidase/MPO Activity Assay Kit (Colorimetric)

• EC 1.11.1
• EC 1.11.1.7
• MPO
• Myeloperoxidase

Background

FUNCTION: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. MPO is an important marker for myeloid cells, from the promyelocyte stage and to the mature forms. CATALYTIC ACTIVITY: Donor + H2O2 = oxidized donor + 2 H2O. CATALYTIC ACTIVITY: Cl- + H2O2 = HOCl + 2 H2O. COFACTOR: Binds 1 calcium ion per heterodimer. COFACTOR: Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer. SUBUNIT: Tetramer of two light chains and two heavy chains. SUBCELLULAR LOCATION: Lysosome. ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. DISEASE: Defects in MPO are the cause of myeloperoxidase deficiency (MPD). MPD is an autosomal recessive defect that results in disseminated candidiasis. SIMILARITY: Belongs to the peroxidase family. XPO subfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: MPO