Myosin Heavy Chain Antibody (MF20) [Unconjugated]

Myosin Heavy Chain was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Myosin Heavy Chain was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Myosin Heavy Chain was detected in perfusion fixed frozen sections of mouse skeletal muscle using Mouse Anti-Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Knockdown of BAMBI inhibited myogenic differentiation. All the cell samples were harvested after transfection and myogenic induction for 48 and 96 h. (a) The western blot images of BAMBI and GAPDH; (b) the efficiency of siRNA interference on the mRNA and protein expression of BAMBI; (c) the mRNA expression of MyoD at 48 h and that of MyoG and MyHC at 96 h; (d) the western blot images of MyoD at 48 h, MyHC at 96 h, and their corresponding GAPDH; (e) the protein expression of MyoD at 48 h and MyHC at 96 h; (f) immunofluorescence of MyHC in C2C12 myotubes at 96 h post differentiation, images captured at 100× magnification; (g) the populations of myotubes; (h) the differentiation index; and (i) the myotube fusion index. The results were represented as mean ± SD; n = 3; * p 4 indicates myotubes with 4 more nucleus; (G,H) The mRNA expression of MyoD, MyoG, MyHC on 3 d and 5 d post differentiation; (I,J) The mRNA expression of Myomarker and Myomixer 3 d and 5 d post differentiation; (K) The statistical results of MyoD, MyoG, MyHC proteins in Figure 2L; (L) Western blot analyzed for MyoD, MyoG, MyHC proteins 5 d post differentiation; (M) Protein levels of key molecules in PI3K-AKT pathway in C2C12 cells transfected with miR-106a-5p agomir or NC on 5 d post differentiation; (N) The statistical analysis of phosphorylated PI3K (p85 alpha ), AKT (sre473) and mTOR (ser2448). Data were presented as mean ± SEM. n = 3 per group. * p MAB004, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Saponin. View our protocol for Staining Intracellular Molecules.

• Reactivity: All-Multi
• Applications: WB, Flow, IHC, ICC/IF
• Clone: MF20
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Myosin Heavy Chain Antibody (MF20) [Unconjugated] Summary

• Immunogen: Chicken pectoralis-derived Myosin
• Specificity: Detects Myosin Heavy Chain in human, mouse, rat and other mammalian, avian, and amphibian species.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MYH14
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Myosin Heavy Chain Antibody (MF20) [Unconjugated]

• DFNA4
• FLJ13881
• FLJ43092
• KIAA2034DKFZp667A1311
• MHC16
• MYH14 variant protein
• MYH17
• MYHC
• Myosin heavy chain 14
• Myosin Heavy Chain
• Myosin heavy chain, non-muscle IIc
• myosin
• myosin, heavy chain 14
• myosin, heavy chain 14, non-muscle
• myosin, heavy polypeptide 14
• myosin-14
• NMHC II-C
• NMHC-II-C
• Non-muscle myosin heavy chain IIc
• nonmuscle myosin heavy chain II-C

Background

Skeletal muscle Myosin or myosin II is the motor protein that generates force to drive muscle contraction. It is a 520 kDa hexamer comprised of two heavy chains and four light chains. Myosin heavy chain is 220 kDa in size and consists of a long coiled-coil domain tail that mediates dimerization of the two heavy chains and a globular head region that mediates ATP-dependent sliding of actin filaments. Myosin heavy chain can be proteolytically cleaved to produce heavy meromyosin, which includes the S1 motor domain (head region) and first third of the coiled coil domain, and light meromyosin, which includes the C-terminal two thirds of the coiled coil domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myosin Heavy Chain Antibody (MAB4470)(168)

Publications using MAB4470

• Silvestri, B;Mochi, M;Mawrie, D;de Turris, V;Colantoni, A;Borhy, B;Medici, M;Anderson, EN;Garone, MG;Zammerilla, CP;Simula, M;Ballarino, M;Pandey, UB;Rosa, A; HuD impairs neuromuscular junctions and induces apoptosis in human iPSC and Drosophila ALS models Nature communications 2024-11-07 [PMID: 39511225] (Immunocytochemistry, Human)
• Yu Liu, S;Chen, LK;Chung, YT;Chen, CW;Wu, GL;Chang, YC;Chen, PR;Chang, YI;Lin, HF;Wu, LY;Juan, CC; Glucosamine inhibits myoblast proliferation and differentiation, and stimulates myotube atrophy through distinct signal pathways The Journal of nutritional biochemistry 2024-09-07 [PMID: 39251145] (Immunocytochemistry, Mouse)
• Maejima, Y;Nah, J;Aryan, Z;Zhai, P;Sung, EA;Liu, T;Takayama, K;Moghadami, S;Sasano, T;Li, H;Sadoshima, J; Mst1-mediated phosphorylation of FoxO1 and C/EBP-? stimulates cell-protective mechanisms in cardiomyocytes Nature communications 2024-07-25 [PMID: 39060225] (Immunocytochemistry, Rat)
• Gao, H;Huang, X;Cai, Z;Cai, B;Wang, K;Li, J;Kuang, J;Wang, B;Zhai, Z;Ming, J;Cao, S;Qin, Y;Pei, D; Generation of musculoskeletal cells from human urine epithelium-derived presomitic mesoderm cells Cell & bioscience 2024-07-15 [PMID: 39010176] (Flow Cytometry, Immunohistochemistry, Human, Mouse)
• Lee, B;Park, Y;Lee, Y;Kwon, S;Shim, J; Triptolide, a Cancer Cell Proliferation Inhibitor, Causes Zebrafish Muscle Defects by Regulating Notch and STAT3 Signaling Pathways International journal of molecular sciences 2024-04-25 [PMID: 38731894] (Immunohistochemistry, Immunocytochemistry, Mouse, Zebrafish)
• Fukushima, T;Hasegawa, Y;Kuse, S;Fujioka, T;Nikawa, T;Masubuchi, S;Sakakibara, I; PHF2 regulates sarcomeric gene transcription in myogenesis PloS one 2024-05-03 [PMID: 38701072] (Immunocytochemistry, Mouse)
• Sun, Y;Ramesh, V;Wei, F;Locasale, JW; Methionine availability influences essential H3K36me3 dynamics during cell differentiation bioRxiv : the preprint server for biology 2023-11-22 [PMID: 38045360] (Western Blot, Mouse)
• Sarsons, CD;Gilham, D;Tsujikawa, LM;Wasiak, S;Fu, L;Rakai, BD;Stotz, SC;Carestia, A;Sweeney, M;Kulikowski, E; Apabetalone, a Clinical-Stage, Selective BET Inhibitor, Opposes DUX4 Target Gene Expression in Primary Human FSHD Muscle Cells Biomedicines 2023-09-30 [PMID: 37893058] (ICC, Human)
• Tetsuhiro Kakimoto, Akira Ogasawara, Kiyoshi Ishikawa, Takashi Kurita, Kumiko Yoshida, Shuichi Harada et al. A Systemically Administered Unconjugated Antisense Oligonucleotide Targeting DUX4 Improves Muscular Injury and Motor Function in FSHD Model Mice Biomedicines 8/22/2023 [PMID: 37760780]
• Yoshioka, Y;Oishi, S;Onoda, K;Shibata, K;Miyoshi, N; Diosgenin prevents dexamethasone-induced myotube atrophy in C2C12?cells Archives of biochemistry and biophysics 2023-09-16 [PMID: 37722527] (Western Blot, ICC, Mouse)
• Aeyung Kim, Sang-Min Park, No Soo Kim, Haeseung Lee Ginsenoside Rc, an Active Component of Panax ginseng, Alleviates Oxidative Stress-Induced Muscle Atrophy via Improvement of Mitochondrial Biogenesis Antioxidants (Basel) 8/7/2023 [PMID: 37627571]
• Jo, SI;Park, YS;Chang, Y;Moon, JH;Lee, S;Lee, H;Kim, M;Kim, DY;Bae, S;Park, SY;Yun, H;You, JE;Im, M;Han, HJ;Kim, SY;Jin, DH; Effects of the purified dry extract of fermented ginseng BST204 on muscle fiber regeneration Biochemistry and biophysics reports 2023-09-01 [PMID: 37601455] (Western Blot, Mouse)
• Das, D;Leung, JY;Tergaonkar, V;Loh, AHP;Chiang, CM;Taneja, R; BRD4 isoforms have distinct roles in tumor progression and metastasis in embryonal rhabdomyosarcoma bioRxiv : the preprint server for biology 2023-07-27 [PMID: 37546805] (Immunocytochemistry, Human)
• Cao, H;Du, T;Li, C;Wu, L;Liu, J;Guo, Y;Li, X;Yang, G;Jin, J;Shi, X; MicroRNA-668-3p inhibits myoblast proliferation and differentiation by targeting Appl1 BMC genomics 2023-07-24 [PMID: 37488537] (Western Blot, Immunocytochemistry, Mouse)
• Matsumoto C, Sekine H, Zhang N et al. Role of p53 in Cisplatin-Induced Myotube Atrophy International Journal of Molecular Sciences 5/24/2023 [PMID: 37298128]
  
  Details:
  Dilution: 10 ?g/mL
• Liao, WC;Chen, CT;Tsai, YS;Wang, XY;Chang, YT;Wu, MS;Chow, LP; S100A8, S100A9 and S100A8/A9 heterodimer as novel cachexigenic factors for pancreatic cancer-induced cachexia BMC cancer 2023-06-06 [PMID: 37280516] (Immunocytochemistry, Mouse)
• Tomoka Katayama, Yuta Chigi, Daiji Okamura The ensured proliferative capacity of myoblast in serum-reduced conditions with Methyl-beta -cyclodextrin Frontiers in Cell and Developmental Biology 5/12/2023 [PMID: 37250904]
• Tao, W;Ouyang, Z;Liao, Z;Li, L;Zhang, Y;Gao, J;Ma, L;Yu, S; Ursolic Acid Alleviates Cancer Cachexia and Prevents Muscle Wasting via Activating SIRT1 Cancers 2023-04-20 [PMID: 37190306] (ICC, Western Blot, Mouse)
• Y Yamashita, S Nakada, K Nakamura, H Sakurai, K Ohno, T Goto, Y Mabuchi, C Akazawa, N Hattori, E Arikawa-Hi Evaluation of Human-Induced Pluripotent Stem Cells Derived from a Patient with Schwartz-Jampel Syndrome Revealed Distinct Hyperexcitability in the Skeletal Muscles Biomedicines, 2023-03-07;11(3):. 2023-03-07 [PMID: 36979792] (IF, Mouse)
• Barbara Tedesco, Leen Vendredy, Elias Adriaenssens, Marta Cozzi, Bob Asselbergh, Valeria Crippa et al. HSPB8 frameshift mutant aggregates weaken chaperone-assisted selective autophagy in neuromyopathies Autophagy 8/3/2023 [PMID: 36854646]
• Huang R, Yamamoto T, Nakata E et al. CDKAL1 Drives the Maintenance of Cancer Stem-Like Cells by Assembling the eIF4F Translation Initiation Complex Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2/14/2023 [PMID: 36786012]
• L Vo, MW Schmidtke, NT Da Rosa-Ju, M Ren, M Schlame, ML Greenberg Cardiolipin metabolism regulates expression of muscle transcription factor MyoD1 and muscle development The Journal of Biological Chemistry, 2023-02-04;0(0):102978. 2023-02-04 [PMID: 36739949] (Western Blot, Transgenic Mouse)
• Watanabe S, Sudo Y, Makino T, Kimura S Skeletal muscle releases extracellular vesicles with distinct protein and miRNA signatures that function in the muscle microenvironment PNAS 1/1/2022 [PMID: 36714847]
• Zhao W, Chen L, Tan W et al. Mannan Oligosaccharides Promoted Skeletal Muscle Hypertrophy through the Gut Microbiome and Microbial Metabolites in Mice Foods 1/12/2023 [PMID: 36673449]
• Zhao W, Chen L, Tan W et al. Mannan Oligosaccharides Promoted Skeletal Muscle Hypertrophy through the Gut Microbiome and Microbial Metabolites in Mice Foods 2023-01-12 [PMID: 36673449]
• K Shintani-I, R Tsurumi, H Ikegaya Decrease in the expression of muscle-specific miRNAs, miR-133a and miR-1, in myoblasts with replicative senescence PLoS ONE, 2023-01-17;18(1):e0280527. 2023-01-17 [PMID: 36649291] (ICC, Western Blot, Mouse)
• Xiangjiao Yi, Jianguo Tao, Yu Qian, Feng Feng, Xueqin Hu, Taotao Xu et al. Morroniside ameliorates inflammatory skeletal muscle atrophy via inhibiting canonical and non-canonical NF-kappa B and regulating protein synthesis/degradation Frontiers in Pharmacology 12/23/2022 [PMID: 36618945]
• L Wei, R Wang, K Lin, X Jin, L Li, J Wazir, W Pu, P Lian, R Lu, S Song, Q Zhao, J Li, H Wang Creatine modulates cellular energy metabolism and protects against cancer cachexia-associated muscle wasting Frontiers in Pharmacology, 2022-12-07;13(0):1086662. 2022-12-07 [PMID: 36569317] (ICC, Western Blot, Mouse)
• L Li, X Zhang, H Yang, X Xu, Y Chen, D Dai, S Zhan, J Guo, T Zhong, L Wang, J Cao, H Zhang miR-193b-3p Promotes Proliferation of Goat Skeletal Muscle Satellite Cells through Activating IGF2BP1 International Journal of Molecular Sciences, 2022-12-12;23(24):. 2022-12-12 [PMID: 36555418] (IHC, Goat)
• J Shen, J Wang, H Zhen, Y Liu, L Li, Y Luo, J Hu, X Liu, S Li, Z Hao, M Li, Z Zhao MicroRNA-381 Regulates Proliferation and Differentiation of Caprine Skeletal Muscle Satellite Cells by Targeting PTEN and JAG2 International Journal of Molecular Sciences, 2022-11-05;23(21):. 2022-11-05 [PMID: 36362373] (IHC, Goat)
• WY Fang, CL Lin, WH Chang, CH Chang, YC Huang, YH Tsai, FR Chang, YC Lo Protective Effects of the Chalcone-Based Derivative AN07 on Inflammation-Associated Myotube Atrophy Induced by Lipopolysaccharide International Journal of Molecular Sciences, 2022-10-26;23(21):. 2022-10-26 [PMID: 36361718] (IHC, Western Blot, Mouse)
• W Chen, W Chen, P Liu, S Qian, S Tao, M Huang, W Xu, C Li, X Chen, H Lin, Z Qin, J Lu, S Xie Role of lncRNA Has2os in Skeletal Muscle Differentiation and Regeneration Cells, 2022-11-04;11(21):. 2022-11-04 [PMID: 36359891] (ICC, Western Blot, Mouse)
• Sungmin Nam, Bo Ri Seo, Alexander J. Najibi, Stephanie L. McNamara, David J. Mooney Active tissue adhesive activates mechanosensors and prevents muscle atrophy Nature Materials 2/1/2023 [PMID: 36357687]
• RW Matheny, AL Kolb, AV Geddis, BM Roberts Celecoxib impairs primary human myoblast proliferation and differentiation independent of cyclooxygenase 2 inhibition Physiological Reports, 2022-11-01;10(21):e15481. 2022-11-01 [PMID: 36325583] (Western Blot, Human)
• Mengistu Lemecha, Jaya Prakash Chalise, Yuki Takamuku, Guoxiang Zhang, Takahiro Yamakawa, Garrett Larson et al. Lcn2 mediates adipocyte-muscle-tumor communication and hypothermia in pancreatic cancer cachexia Molecular Metabolism 12/1/2022 [PMID: 36243318]
• L Wei, R Wang, J Wazir, K Lin, S Song, L Li, W Pu, C Zhao, Y Wang, Z Su, H Wang 2-Deoxy-D-glucose Alleviates Cancer Cachexia-Induced Muscle Wasting by Enhancing Ketone Metabolism and Inhibiting the Cori Cycle Cells, 2022-09-25;11(19):. 2022-09-25 [PMID: 36230949] (ICC, Western Blot, Mouse)
• Xiaofan Yang, Pingping Xue, Zhenyu Liu, Wenqing Li, Chuyan Li, Zhenbing Chen SESN2 prevents the slow-to-fast myofiber shift in denervated atrophy via AMPK/PGC-1 alpha pathway Cellular & Molecular Biology Letters 12/1/2022 [PMID: 35945510]
• Cathrine Nordgaard, Anna Constance Vind, Amy Stonadge, Rasmus Kjøbsted, Goda Snieckute, Pedro Antas et al. ZAK beta is activated by cellular compression and mediates contraction‐induced MAP kinase signaling in skeletal muscle The EMBO Journal 9/1/2022 [PMID: 35899396]
• Farogh Ahsan, Tarique Mahmood, Tanveer A. Wani, Seema Zargar, Mohammed Haris Siddiqui, Shazia Usmani et al. Effectual Endeavors of Silk Protein Sericin against Isoproterenol Induced Cardiac Toxicity and Hypertrophy in Wistar Rats Life (Basel) 7/15/2022 [PMID: 35888151]
• M Ling, X Lai, L Quan, F Li, L Lang, Y Fu, S Feng, X Yi, C Zhu, P Gao, X Zhu, L Wang, G Shu, Q Jiang, S Wang Knockdown of VEGFB/VEGFR1 Signaling Promotes White Adipose Tissue Browning and Skeletal Muscle Development International Journal of Molecular Sciences, 2022-07-07;23(14):. 2022-07-07 [PMID: 35886871] (Western Blot, Mouse)
• ZZ He, T Zhao, N Qimuge, T Tian, W Yan, X Yi, J Jin, R Cai, T Yu, G Yang, W Pang COPS3 AS lncRNA enhances myogenic differentiation and maintains fast-type myotube phenotype Cellular Signalling, 2022-04-28;95(0):110341. 2022-04-28 [PMID: 35491006] (ICC/IF, Mouse)
• B Kyei, E Odame, L Li, L Yang, S Zhan, J Li, Y Chen, D Dai, J Cao, J Guo, T Zhong, L Wang, H Zhang Knockdown of CDR1as Decreases Differentiation of Goat Skeletal Muscle Satellite Cells via Upregulating miR-27a-3p to Inhibit ANGPT1 Genes, 2022-04-09;13(4):. 2022-04-09 [PMID: 35456469] (ICC, Goat)
• Ricardo Ferrari, Bowen Xie, Edwyn Assaf, Kristin Morder, Melanie Scott, Hong Liao et al. Inflammatory Caspase Activity Mediates HMGB1 Release and Differentiation in Myoblasts Affected by Peripheral Arterial Disease Cells 3/30/2022 [PMID: 35406727]
• I Kim, A Ghosh, N Bundschuh, L Hinte, E Petrosyan, F von Meyenn, O Bar-Nur Integrative molecular roadmap for direct conversion of fibroblasts into myocytes and myogenic progenitor cells Science Advances, 2022-04-06;8(14):eabj4928. 2022-04-06 [PMID: 35385316] (ICC/IF, Mouse)
• A Sawada, T Yamamoto, T Sato Tceal5 and Tceal7 Function in C2C12 Myogenic Differentiation via Exosomes in Fetal Bovine Serum International Journal of Molecular Sciences, 2022-02-12;23(4):. 2022-02-12 [PMID: 35216152] (ICC, Human)
• Tomoki Sakakida, Takeshi Ishikawa, Toshifumi Doi, Ryuichi Morita, Yuki Endo, Shinya Matsumura et al. Water‐soluble dietary fiber alleviates cancer‐induced muscle wasting through changes in gut microenvironment in mice Cancer Science 5/1/2022 [PMID: 35201655]
• Fumi SATOH, Akihiro SUGIURA, Jiro TASHIRO, Yoshinao Z. HOSAKA, Katsuhiko WARITA Chondroitin sulfate E downregulates N-cadherin and suppresses myotube formation Journal of Veterinary Medical Science 4/13/2022 [PMID: 35173094]
• Y Li, W Chen, K Ogawa, M Koide, T Takahashi, Y Hagiwara, E Itoi, T Aizawa, M Tsuchiya, R Izumi, N Suzuki, M Aoki, M Kanzaki Feeder-supported in vitro exercise model using human satellite cells from patients with sporadic inclusion body myositis Scientific Reports, 2022-01-20;12(1):1082. 1/20/2022 [PMID: 35058512]
• M Ling, L Quan, X Lai, L Lang, F Li, X Yang, Y Fu, S Feng, X Yi, C Zhu, P Gao, X Zhu, L Wang, G Shu, Q Jiang, S Wang VEGFB Promotes Myoblasts Proliferation and Differentiation through VEGFR1-PI3K/Akt Signaling Pathway International Journal of Molecular Sciences, 2021-12-12;22(24):. 2021-12-12 [PMID: 34948148] (ICC, Western Blot, Mouse)
• MF Coleman, KA Liu, AJ Pfeil, SK Etigunta, X Tang, S Fabela, LM Lashinger, Z Cui, SD Hursting beta-Hydroxy-beta-Methylbutyrate Supplementation Promotes Antitumor Immunity in an Obesity Responsive Mouse Model of Pancreatic Ductal Adenocarcinoma Cancers, 2021-12-18;13(24):. 2021-12-18 [PMID: 34944981] (Western Blot, Mouse)
• Lu Li, Huiquan Liu, Weili Tao, Su Wen, Xiaofen Fu, Shiying Yu Pharmacological Inhibition of HMGB1 Prevents Muscle Wasting Frontiers in Pharmacology 11/18/2021 [PMID: 34867338]
• K Xie, H Xiong, W Xiao, Z Xiong, W Hu, J Ye, N Xu, J Shi, C Yuan, Z Chen, D Miao, X Zhang, H Yang Downregulation of miR-29c promotes muscle wasting by modulating the activity of leukemia inhibitory factor in lung cancer cachexia Cancer Cell International, 2021-11-27;21(1):627. 2021-11-27 [PMID: 34838029] (ICC, Mouse)
• X Liu, E Zu, X Chang, X Ma, Z Wang, X Song, X Li, Q Yu, KI Kamei, T Hayashi, K Mizuno, S Hattori, H Fujisaki, T Ikejima, DO Wang Bi-phasic effect of gelatin in myogenesis and skeletal muscle regeneration Disease Models & Mechanisms, 2021-12-24;14(12):. 2021-12-24 [PMID: 34821368] (ICC, Western Blot, Mouse)
• RH Mennen, N Hallmark, M Pallardy, R Bars, H Tinwell, AH Piersma Gene regulation by morpholines and piperidines in the cardiac embryonic stem cell test Toxicology and Applied Pharmacology, 2021-10-29;433(0):115781. 2021-10-29 [PMID: 34737147] (ICC, Mouse)
• Evelin Major, Ilka Keller, Dániel Horváth, István Tamás, Ferenc Erdődi, Beáta Lontay Smoothelin-Like Protein 1 Regulates the Thyroid Hormone-Induced Homeostasis and Remodeling of C2C12 Cells via the Modulation of Myosin Phosphatase International Journal of Molecular Sciences 9/24/2021 [PMID: 34638630]
• M Zhou, B Li, C Liu, M Hu, J Tang, J Min, J Cheng, L Hong M2 Macrophage-derived exosomal miR-501 contributes to pubococcygeal muscle regeneration International immunopharmacology, 2021-10-08;101(0):108223. 2021-10-08 [PMID: 34634686] (ICC, IHC, Mouse)
• SE Park, JB Jeong, SJ Oh, SJ Kim, H Kim, A Choi, SJ Choi, SY Oh, GH Ryu, J Lee, HB Jeon, JW Chang Wharton's Jelly-Derived Mesenchymal Stem Cells Reduce Fibrosis in a Mouse Model of Duchenne Muscular Dystrophy by Upregulating microRNA 499 Biomedicines, 2021-08-26;9(9):. 2021-08-26 [PMID: 34572277] (IHC, Western Blot, Mouse)
• Hammers DW, Hart CC, Matheny MK et al. Filopodia powered by class x myosin promote fusion of mammalian myoblasts eLife 2021-09-14 [PMID: 34519272]
• Hammers DW, Hart CC, Matheny MK et al. Filopodia powered by class x myosin promote fusion of mammalian myoblasts eLife 9/14/2021 [PMID: 34519272]
• GW Tan, T Kondo, K Imamura, M Suga, T Enami, A Nagahashi, K Tsukita, I Inoue, J Kawaguchi, T Shu, H Inoue Simple derivation of skeletal muscle from human pluripotent stem cells using temperature-sensitive Sendai virus vector Journal of Cellular and Molecular Medicine, 2021-09-12;0(0):. 2021-09-12 [PMID: 34510713] (ICC, Human)
• Rui Chen, Si Lei, Yanling She, Shanyao Zhou, Huacai Shi, Cheng Li et al. Lnc-GD2H Promotes Proliferation by Forming a Feedback Loop With c-Myc and Enhances Differentiation Through Interacting With NACA to Upregulate Myog in C2C12 Myoblasts Frontiers in Cell and Developmental Biology 8/18/2021 [PMID: 34490239]
• E Ichimura, K Ojima, S Muroya, T Suzuki, K Kobayashi, T Nishimura The ubiquitin ligase Ozz decreases the replacement rate of embryonic myosin in myofibrils Physiological Reports, 2021-09-01;9(17):e15003. 2021-09-01 [PMID: 34435451] (Immunoprecipitation, Western Blot, Mouse)
• RH Mennen, N Hallmark, M Pallardy, R Bars, H Tinwell, AH Piersma Molecular neural crest cell markers enable discrimination of organophosphates in the murine cardiac embryonic stem cell test Toxicology reports, 2021-07-31;8(0):1513-1520. 2021-07-31 [PMID: 34401361] (ICC, Mouse)
• Y She, C Li, T Jiang, S Lei, S Zhou, H Shi, R Chen Knockdown of CNN3 Impairs Myoblast Proliferation, Differentiation, and Protein Synthesis via the mTOR Pathway Frontiers in Physiology, 2021-07-08;12(0):659272. 2021-07-08 [PMID: 34305633] (Western Blot, Mouse)
• B Lee, S Lee, Y Lee, Y Park, J Shim Emerin Represses STAT3 Signaling through Nuclear Membrane-Based Spatial Control International Journal of Molecular Sciences, 2021-06-22;22(13):. 2021-06-22 [PMID: 34206382] (IHC, Mouse)
• M Niu, L Li, Z Su, L Wei, W Pu, C Zhao, Y Ding, J Wazir, W Cao, S Song, Q Gao, H Wang An integrative transcriptome study reveals Ddit4/Redd1 as a key regulator of cancer cachexia in rodent models Cell Death & Disease, 2021-06-26;12(7):652. 2021-06-26 [PMID: 34175899] (IHC, Mouse)
• K Yoshioka, H Nagahisa, F Miura, H Araki, Y Kamei, Y Kitajima, D Seko, J Nogami, Y Tsuchiya, N Okazaki, A Yonekura, S Ohba, Y Sumita, K Chiba, K Ito, I Asahina, Y Ogawa, T Ito, Y Ohkawa, Y Ono Hoxa10 mediates positional memory to govern stem cell function in adult skeletal muscle Science Advances, 2021-06-09;7(24):. 2021-06-09 [PMID: 34108202] (IHC, Mouse)
• Yujie Zhang, Yuxin Zhang, Yichen Li, Li Zhang, Shiying Yu Preclinical Investigation of Alpinetin in the Treatment of Cancer-Induced Cachexia via Activating PPAR gamma Frontiers in Pharmacology 5/21/2021 [PMID: 34093209]
• A Moustogian, A Philippou, O Taso, E Zevolis, M Pappa, A Chatzigeor, M Koutsilier The Effects of Muscle Cell Aging on Myogenesis International Journal of Molecular Sciences, 2021-04-02;22(7):. 2021-04-02 [PMID: 33918414] (ICC, Mouse)
• Naoki Ito, Yuko Miyagoe-Suzuki, Shin’ichi Takeda, Akira Kudo Periostin Is Required for the Maintenance of Muscle Fibers during Muscle Regeneration International Journal of Molecular Sciences 3/31/2021 [PMID: 33807264]
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Reviews for Myosin Heavy Chain Antibody (MAB4470) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Patricia Carulla Martí ICC Human 06/19/2023

Summary

• Application: Immunocytochemistry
• Sample Tested: Primary human myocytes
• Species: Human
• Lot: CAEI0817071

reviewed by: Zachary Patinkin Simple Western Human 02/25/2015

Summary

• Application: Simple Western
• Sample Tested: Human Skeletal Muscle Lysate; 3T3-L1 cell lysate
• Species: Human
• Lot: CAEI0314061

Bioinformatics

• Gene Symbol: MYH14