Myosin VB Antibody

Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-50217] - Analysis of anti-MYO5B antibody with human skeletal muscle at concentration 5 ug/ml.

Product Discontinued

Myosin VB Antibody Summary

• Immunogen: Synthetic peptide from human Myosin VB.
• Specificity: Synthetic peptide corresponding to N-terminal residues of human Myosin VB (Myosin-5b).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MYO5B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1 ug/ml
• Application Notes: IHC: NBP1-50217 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Myosin VB Antibody

• KIAA1119
• MYO5B variant protein
• myosin VB
• myosin-Vb

Background

Myosin and Kinesin motor domain. These ATPases belong to the P-loop NTPase family and provide the driving force in myosin and kinesin mediated processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MYO5B
• Entrez:
  • 4645(Human)
• Uniprot:
  • Q9ULV0(Human)