myosin X Antibody

Western Blot: myosin X Antibody [22430002] - Detection of Myo10 in HeLa cells at a dilution of 1:800 with 1 mg/mL stock. The predicted molecular weight of MYO10, based on the amino acid sequence, is 237 kD. MYO10 appeared at approximately 250 kD on this gel.

Immunocytochemistry/Immunofluorescence: myosin X Antibody [22430002] - The effect of HOXA5 on single cell migration. The median speed was calculated by tracking the movement of individual cells with around 25 cells per assay condition in three independent experiments. *, P<0.05 compared with the mock control. Representative photographs of immunofluorescence staining of endogenous F-actin in stably expressing HOXA5 or control vector cells (Mock). Cells were fixed and stained for Myosin-X. Nuclei were counterstained with DAPI. Merge images were also shown. Scale bars, 20 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0124191), licensed under a CC-BY license.

Immunohistochemistry: myosin X Antibody [22430002] - Most of the normal tissues showed moderate cytoplasmic positivity. Strong staining was observed in stomach, rectum, exocrine pancreas, urothelium and in placenta. Hepatocytes were negative. Malignant tissues exhibited similar staining properties as normal cells. Occasional tumor cases displayed strong cytoplasmic immunoreactivity. Most cases of malignant lymphoma, testicular and prostate cancers were negative or weakly stained. Image and statement courtesy of the Human Protein Atlas (HPA).

Immunocytochemistry/Immunofluorescence: myosin X Antibody [22430002] - staining of Actin at a dilution of 1:800. Image courtesy of MM DiVito and RE Cheney, Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill.

Immunocytochemistry/Immunofluorescence: myosin X Antibody [22430002] - Myosins are actin-based motor molecules with ATPase activity. MYO10 plays a role in regions of dynamic actin and is presumed to serve in intracellular movements. Immunofluorescence staining of Myo10 at a dilution of 1:800 with 1.0 mg/mL stock. Image courtesy of MM DiVito and RE Cheney, Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill.

Immunocytochemistry/Immunofluorescence: myosin X Antibody [22430002] - staining of Myo10 at a dilution of 1:800 with 1.0mg/mL stock overlapped with immunofluorescence staining of Actin at a dilution of 1:800. Image courtesy of MM DiVito and RE Cheney, Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill.

ELISA: myosin X Antibody [22430002]

Product Discontinued

myosin X Antibody Summary

• Immunogen: This antibody is specific for the Middle Region of the target protein (NM_012334).
• Specificity: This product is specific for Human Myosin 10.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MYO10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:800
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is useful in ELISA, Immunofluorescence, Immunohistochemistry-Paraffin and Western Blot.

Reactivity Notes

Human. mouse reactivity reported in scientific literature (PMID: 24270889)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for myosin X Antibody

• FLJ10639
• FLJ21066
• FLJ22268
• KIAA0799FLJ43256
• MGC131988
• myosin X
• myosin-X
• Unconventional myosin-10

Background

Myosin motor domain. This catalytic (head) domain has ATPase activity and belongs to the larger group of P-loop NTPases. Myosins are actin-dependent molecular motors that play important roles in muscle contraction, cell motility.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for myosin X Antibody (22430002)(16)

Publications using 22430002

• Popovi? A, Miihkinen M, Ghimire S et al. Myosin-X recruits lamellipodin to filopodia tips Journal of cell science 2023-03-01 [PMID: 36861887] (Western Blot, Human)
• Adebowale K, Gong Z, Hou JC et al. Enhanced substrate stress relaxation promotes filopodia-mediated cell migration Nature materials 2021-04-19 [PMID: 33875851]
• Young LE, Latario CJ, Higgs HN. Roles for Ena/VASP proteins in FMNL3-mediated filopodial assembly. J. Cell. Sci. 2018-10-29 [PMID: 30373894] (WB, Human)
• Lehtimaki JI, Fenix AM, Kotila TM et al. UNC-45a promotes myosin folding and stress fiber assembly. J. Cell Biol. 2017-10-20 [PMID: 29055011] (WB)
• Jacquemet G, Baghirov H, Georgiadou M et al. L-type calcium channels regulate filopodia stability and cancer cell invasion downstream of integrin signalling. Nat Commun. 2016-12-02 [PMID: 27910855] (WB, Human)
• Margiotta A, Progida C, Bakke O, Bucci C. Rab7a regulates cell migration through Rac1 and vimentin. Biochim Biophys Acta 2017-02-01 [PMID: 27888097] (Human)
• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Osswald M, Jung E, Sahm F et al. Brain tumour cells interconnect to a functional and resistant network. Nature. 2015-12-03 [PMID: 26536111] (ICC/IF)
• Wang CC, Su KY, Chen HY et al. HOXA5 Inhibits Metastasis via Regulating Cytoskeletal Remodelling and Associates with Prolonged Survival in Non-Small-Cell Lung Carcinoma PLoS ONE 2015-04-16 [PMID: 25875824] (ICC/IF, Human)
• Wang Y, Brooks PJ, Jang JJ et al. Role of actin filaments in fusopod formation and osteoclastogenesis Biochim. Biophys. Acta 2015-04-12 [PMID: 25871908] (ICC/IF, Mouse)
• Arjonen A, Kaukonen R, Mattila E et al. Mutant p53-associated myosin-X upregulation promotes breast cancer invasion and metastasis. J. Clin. Invest. 2014-02-03 [PMID: 24487586] (WB, IHC-P, Human, Mouse)
• Fierro-Gonzalez JC, White MD, Silva JC, Plachta N. Cadherin-dependent filopodia control preimplantation embryo compaction. Nat Cell Biol. 2013-12-01 [PMID: 24270889] (IF/IHC, ICC/IF, Mouse)
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.
• Tseng P, Judy JW, Di Carlo D. Magnetic nanoparticle-mediated massively parallel mechanical modulation of single-cell behavior Nat Methods 2012-10-14 [PMID: 23064517] (ICC/IF, Human)
• Miihkinen M, GrOnloh M, Vihinen H et al. Myosin-X is required for integrin activation at filopodia tips bioRxiv 2020-05-06 (WB, Human)
• Muscarella AM, Dai W, Mitchell PG et al. Unique cellular protrusions mediate breast cancer cell migration by tethering to osteogenic cells NPJ breast cancer 2020-09-07 [PMID: 32964116] (ICC/IF, Human, Mouse)

FAQs for myosin X Antibody (22430002). (Showing 1 - 1 of 1 FAQs).

1. Can you tell me the antigen sequence of Myosin 10 Antibody (22430002).
   • This is a product that there is not additional immunogen sequence information available for, as it is deemed proprietary.

Bioinformatics

• Gene Symbol: MYO10
• Entrez:
  • 4651(Human)
• Uniprot:
  • Q9HD67(Human)