Nanog Antibody

Western Blot: Nanog Antibody [29290002] - Cell lysate from 293 over expressing Nanog was subjected to SDS-PAGE and transfer to nitrocellulose membrane. The membrane was incubated with anti-Nanog antibody (1:1000 dilution) and then probed anti-rabbit IgG-HRP. Signals were acquired with a CCD camera. Arrow points to unmodified Nanog. The band above the arrow is non-specific. Data courtesy of a SDI customer.

Dot Blot: Nanog Antibody [29290002] - The anti- Nanog antibody (Left quadruplicate spots at each panel) or control antibody (right quadruplicate spots at each panel) were printed on membranes at 10 ng/spot. The membranes were incubated with biotinylated cell lysate (50 ug per membrane), followed by probing with streptavidin-HRP and CCD camera image acquisition. (a), Cell lysate from undifferentiated mouse embryonic stem cells; (b), Cell lysate from differentiated mouse embryonic stem cells. Data courtesy of a SDI customer.

Dot Blot: Nanog Antibody [29290002] - Protein samples (upper left quadruplicate spots: bovine gamma globulin; upper right quadruplicate spots: cell lysate from 293 cell over expressing Nanog; below left quadruplicate spots: cell lysate from 293 cell over expressing Sox2; below right quadruplicate spots: cell lysate from 293 cell over expressing POU5F1) were printed on membranes at 10 ng/spot. The membrane was incubated with anti- Nanog antibody (1:1000 dilution) and then probed anti-rabbit IgG-HRP. Signals were acquired with a CCD camera. Data courtesy of a SDI customer.

ELISA: Nanog Antibody [29290002]

Product Discontinued

Nanog Antibody Summary

• Immunogen: This antibody is specific for the N Terminus Region of human Nanog.
• Marker: Embryonic Stem Cell Marker
• Specificity: This product is specific for human Nanog.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NANOG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: This antibody is useful in ELISA, Immunohistochemistry-Paraffin and Western Blot.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for Nanog Antibody

• FLJ12581
• FLJ40451
• hNanog
• homeobox protein NANOG
• Homeobox transcription factor Nanog
• homeobox transcription factor Nanog-delta 48
• Nanog homeobox
• Nanog

Background

Nanog is a newly identified homeodomain-bearing transcriptional factor. Nanog expression is specific to early embryos and pluripotential stem cells including mouse and human embryonic stem (ES) and embryonic germ (EG) cells. It is a key molecule involved in the signaling pathway for maintaining the capacity for self-renewal and pluripotency, bypassing regulation by the STAT3 pathway. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage. Thus it is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human. NANOG is a new marker for testicular carcinoma in situ and germ cell tumors. Nanog is a 304 amino acid polypeptide which lacks a signal sequence for secretion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nanog Antibody (29290002)(2)

Publications using 29290002

• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

Bioinformatics

• Gene Symbol: NANOG
• Entrez:
  • 79923(Human)
• Uniprot:
  • Q9H9S0(Human)