Recombinant Human Nanog His Protein

Western Blot: Nanog Protein [NBP1-42463] - This protein was tested using the anti-Nanog antibody NB100-93546. 500 ng of protein was used on the western blot and detected with NB100-93546.

SDS-Page: Nanog Protein [NBP1-42463] - The Purity of this product is >95% on a 4-20% SDS-PAGE gel with 3 ug of protein loaded.

Product Discontinued

Recombinant Human Nanog His Protein Summary

• Description: Recombinant Nanog protein with a His tag corresponding to amino acids 1-305.

  Source: E. coli

  (Accession #: Q9H9S0)

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: NANOG
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
  • Western Blot
• Application Notes: This Nanog Protein has a Purity >95%. This product was tested in Western Blot using NB100-93546. The antibody was used to detect 500 ng on the western blot.
  
  Homeobox protein NANOG, Human, His-tagged, Recombinant, E. coli
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 25 mM Tris pH 8.0, 250 mM NaCl, 8 M Urea, 1 mM DTT
• Preservative: N/A
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Nanog His Protein

• FLJ12581
• FLJ40451
• hNanog
• homeobox protein NANOG
• Homeobox transcription factor Nanog
• homeobox transcription factor Nanog-delta 48
• Nanog homeobox
• Nanog

Background

A transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal, NANOG imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Human NANOG with an N-terminal His tag expressed in E. coli and purified by conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NANOG