NCAM-1/CD56 Antibody (123C3.D5)

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Analysis of NCAM-1/CD56 antibody (123C3.D5) on Human colon tissue. Incubation: 1 ug/ml for 30 minutes at room temperature. Image from verified customer review.

Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Flow cytometry of lymphocyte gated PBMCs unstained (gray) orstained with CF568-labeled NCAM-1/CD56 antibody (123C3.D5) (orange).

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Formalin-fixed, paraffin-embedded human colon Ganglion stained with CD56 Monoclonal Antibody (123C3.D5)

Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Analysis using the Azide and BSA Free version of NBP2-15184. Staining of AF700 conjugated CD56 in human PBMC using anti-CD56 antibody. The primary antibody was used at a dilution of 1:100, incubated for 25 min at 4C in 2% human serum, 0.5 mM EDTA in DPBS. Image from verified customer review.

Simple Western: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Simple Western lane view shows a specific band for NCAM-1/CD56 in 0.2 mg/ml of IMR-32 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Electropherogram image of the corresponding Simple Western lane. NCAM-1/CD56 antibody was used at 10 ug/ml dilution of IMR-32 lysates(s) respectively.

• Reactivity: Hu, Rt
• Applications: Simple Western, Flow, ICC/IF, IHC
• Clone: 123C3.D5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

NCAM-1/CD56 Antibody (123C3.D5) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33132)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Membrane preparation of a small cell lung carcinoma
• Localization: Cell surface
• Marker: Neuronal Cell Marker
• Specificity: This monoclonal antibody reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NCAM1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow (Cell Surface)
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Simple Western 10 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
  In Simple Western only 10 - 15 ul of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in IMR-32 lysate(s), separated by Size, antibody dilution of 10 ug/mL, apparent MW was 183 kDa.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for NCAM-1/CD56 Antibody (123C3.D5)

• CD56 / NCAM-1
• CD56 antigen
• CD56
• MSK39
• NCAM1
• N-CAM-1
• NCAM-1
• NCAMantigen recognized by monoclonal 5.1H11
• neural cell adhesion molecule 1
• neural cell adhesion molecule, NCAM

Background

CD56, a 175-220KDa glycoprotein, is a member of the Ig super family. It is expressed as three major isoforms and consists of five Ig-like domains and two Fibronectin-type III domains in the extracellular region. The 135kDa isoform is the basic molecule which is glycosylated or sialylated to produce the mature species. CD56 is widely expressed in nervous system, on NK cells and a specific set of Tcells. CD56+ NK cells and Tcells are unique in their ability to mediate cell-mediated cytotoxicity against certain tumor cell targets without MHC restriction. Other physiological functions of CD56 include mediating cell adhesion through homophilic and heterophilic interaction and activating intracellular signaling pathways resulting in neutrite extension and fasciculation, migration and synapses formation in brain. CD56 is also vital for neuronal development and plasticity in adult brain. It is used as a tumor marker in various cancers such as NK lymphomas and Merkel cell carcinoma. NCAM is expressed on most neuroectodermal derived lines, tissues, and neoplasms such as retinoblastoma, medulloblastoma, astrocytoma, and neuroblastoma. It is also expressed on some mesodermally derived tumors such as rhabdomyosarcoma and also on natural killer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NCAM-1/CD56 Antibody (NBP2-15184)(8)

Publications using NBP2-15184

• Cabral P, Chinn M, Mack J et al. Psychosocial and Cultural Processes Underlying the Epidemiological Paradox within U.S. Latino Sexual Risk: A Systematic Review Behavioral Sciences 2023-03-05 [PMID: 36975251]
• Jarosch S, KOhlen J, Sarker R Et al. Multiplexed imaging and automated signal quantification in formalin-fixed paraffin-embedded tissues by ChipCytometry Cell Rep Methods 2022-04-27 [PMID: 35475000]
• Foy NJ, Forbes KC, Crooke AM et al. Dual Lewis Acid/Photoredox-Catalyzed Addition of Ketyl Radicals to Vinylogous Carbonates in the Synthesis of 2,6-Dioxabicyclo[3.3.0]octan-3-ones. Organic letters 2019-03-04 [PMID: 30188722]
• Lassiter BP, Guo S, Beebe SJ. Nano-Pulse Stimulation Ablates Orthotopic Rat Hepatocellular Carcinoma and Induces Innate and Adaptive Memory Immune Mechanisms that Prevent Recurrence. Cancers (Basel) 2018-03-13 [PMID: 29533981] (Rat)
  
  Details:
  This citation used the Azide and BSA Free version of this antibody.
• Du Z, Bondarenko O, Wang D et al. Ex Vivo Assay of Electrical Stimulation to Rat Sciatic Nerves: Cell Behaviors and Growth Factor Expression. J. Cell. Physiol. 2015-10-30 [PMID: 26516696] (IHC-P, Rat)
• Mooi WJ, Wagenaar SS, Schol D, Hilgers J. Monoclonal antibody 123C3 in lung tumour classification. Immunohistology of 358 resected lung tumours. Mol Cell Probes. 1988-03-01 [PMID: 2837665]
• Schol DJ, Mooi WJ, van der Gugten AA et al. Monoclonal antibody 123C3, identifying small cell carcinoma phenotype in lung tumours, recognizes mainly, but not exclusively, endocrine and neuron-supporting normal tissues. Int J Cancer Suppl. 1988-01-01 [PMID: 2832332]
• Brezicka FT, Olling S, Bergman B et al. Immunohistochemical detection of two small cell lung carcinoma-associated antigens defined by MAbs F12 and 123C3 in bronchoscopy biopsy tissues. APMIS. 1991-09-01 [PMID: 1654059]

Reviews for NCAM-1/CD56 Antibody (NBP2-15184) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Verified Customer IHC-P Human 02/12/2022

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Colon tissue
• Species: Human
• Lot: 123C3.D5

Comments

• Comments: Incubation: 1 ug/ml for 30 minutes at room temperature.

reviewed by: Verified Customer IF Mouse 09/21/2017

Summary

• Application: Immunofluorescence
• Sample Tested: Cortical neurons
• Species: Mouse
• Lot: 4684-1P161212

Bioinformatics

• Gene Symbol: NCAM1
• Uniprot:
  • P13592(Human)
  • P13591(Rat)