NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132] - Formalin-fixed paraffin-embedded human colon Ganglion stained with CD56 Monoclonal antibody (123C3.D5)

Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132] - Flow cytometry of lymphocyte gated PBMCs unstained (gray) orstained with CF568-labeled NCAM-1/CD56 antibody (123C3.D5) (orange).

Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132] - Analysis of AF700 conjugated CD56 in human PBMC using anti-CD56 antibody. The primary antibody was used at a dilution of 1:100, incubated for 25 min at 4C in 2% human serum, 0.5 mM EDTA in DPBS. Image from verified customer review.

Simple Western: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132] - Simple Western lane view shows a specific band for NCAM-1/CD56 in 0.2 mg/ml of IMR-32 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132] - Electropherogram image of the corresponding Simple Western lane. NCAM-1/CD56 antibody was used at 10 ug/ml dilution of IMR-32 lysates(s) respectively.

• Reactivity: Hu, Rt
• Applications: Simple Western, Flow, ICC/IF, IHC
• Clone: 123C3.D5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-15184).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Membrane preparation of a small cell lung carcinoma
• Localization: Cell surface
• Marker: Neuronal Cell Marker
• Specificity: This monoclonal antibody reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NCAM1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow (Cell Surface)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Simple Western
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
  In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free

• CD56 / NCAM-1
• CD56 antigen
• CD56
• MSK39
• NCAM1
• N-CAM-1
• NCAM-1
• NCAMantigen recognized by monoclonal 5.1H11
• neural cell adhesion molecule 1
• neural cell adhesion molecule, NCAM

Background

NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig) like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NCAM-1/CD56 Antibody (NBP2-33132)(2)

Publications using NBP2-33132

• Jarosch S, KOhlen J, Sarker R Et al. Multiplexed imaging and automated signal quantification in formalin-fixed paraffin-embedded tissues by ChipCytometry Cell Rep Methods 2022-04-27 [PMID: 35475000]
• Lassiter BP, Guo S, Beebe SJ. Nano-Pulse Stimulation Ablates Orthotopic Rat Hepatocellular Carcinoma and Induces Innate and Adaptive Memory Immune Mechanisms that Prevent Recurrence. Cancers (Basel) 2018-03-13 [PMID: 29533981] (Rat)
  
  Details:
  This citation used the Azide and BSA Free version of this antibody.

Bioinformatics

• Gene Symbol: NCAM1