NDRG1 Antibody

Western Blot: NDRG1 Antibody [NB300-839] - (0.1ug/ml) staining of Human Kidney (A), Cerebellum (B) and Testis (C) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: NDRG1 Antibody [NB300-839] - staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: NDRG1 Antibody [NB300-839] - (0.5ug/ml) staining of paraffin embedded Human hepatocelluar carcinoma. Data obtained from C. Furer, Visceral Surgery, University of Bern, Switzerland.

Immunohistochemistry-Paraffin: NDRG1 Antibody [NB300-839] - staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NDRG1 Antibody Summary

• Immunogen: Peptide with sequence GNSAGPKSMEVSC corresponding to C-Terminus according to NP_006087.2, NP_001128714.1.
• Specificity: The variants (NP_006087.2; NP_001128714.1) encode the same protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NDRG1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.5 - 2 ug/ml
  • Immunohistochemistry-Paraffin 0.5-2 ug/ml
  • Peptide ELISA detection limit 1:32000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx 48kDa band observed in Human Kidney, and approx 50kDa band observed in Human Brain (Cerebellum) and Testis lysates (calculated MW of 42.8kDa according to NP_006087.2). IHC-P: Human hepatocelluar carcinoma shows strong labeling of membranes and weaker staining of cyrtoplasm. These observations are consistent with those obtained by different antibodies.
• Control:

  NDRG1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NDRG1 Antibody

• CAP43
• CMT4D
• Differentiation-related gene 1 protein
• DRG1
• DRG-1
• DRG1HMSNL
• GC4
• HMSNL
• NDR1
• NDRG1
• Nickel-specific induction protein Cap43
• NMSL
• N-myc downstream regulated 1
• N-myc downstream-regulated gene 1 protein
• protein NDRG1
• protein regulated by oxygen-1
• PROXY1
• Reducing agents and tunicamycin-responsive protein
• RIT42
• RTP
• RTPGC4
• TARG1
• TDD5
• tunicamycin-responsive protein

Background

This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. Mutation in this gene has been reported to be causative for hereditary motor and sensory neuropathy-Lom. May have a growth inhibitory role. Ubiquitous; expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NDRG1 Antibody (NB300-839)(1)

Publication using NB300-839

• Zhou D, Salnikow K, Costa M. Cap43, a novel gene specifically induced by Ni2+ compounds. Cancer Res 1998-05-15 [PMID: 9605764]

Bioinformatics

• Gene Symbol: NDRG1
• Entrez:
  • 10397(Human)
  • 17990(Mouse)
• Uniprot:
  • NP_006087.(Human)