Neuregulin-1/NRG1 Antibody (NRG1/2752)

Immunocytochemistry/Immunofluorescence: Neuregulin-1/NRG1 Antibody (NRG1/2752) [NBP2-79713] - Confocal immunofluorescence of PFA-fixed MCF-7 cells using Neuregulin-1/NRG1 Antibody (NRG1/2752) followed by Goat-anti Mouse CF488 (green) and RedDot is used to label the nuclei red.

Protein Array: Neuregulin-1/NRG1 Antibody (NRG1/2752) [NBP2-79713] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Neuregulin-1/NRG1 Antibody (NRG1/2752). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5

• Reactivity: Hu
• Applications: ELISA, ICC/IF, MA
• Clone: NRG1/2752
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Neuregulin-1/NRG1 Antibody (NRG1/2752) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79847)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant fragment of human Neuregulin-1/NRG1 protein (around aa 21-242) (exact sequence is proprietary) (Uniprot: Q02297)
• Localization: Cell Surface, Cytoplasmic (Secreted)
• Marker: Breast and Urothelial Marker
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: NRG1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Protein Array
• Application Notes: ELISA: For coating, order Ab without BSA.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Neuregulin-1/NRG1 Antibody (NRG1/2752)

• ARIA
• GGF2
• GGFglial growth factor
• Heregulin-1
• HGL
• HGLneu differentiation factor
• HRG
• HRG1
• HRG1-alpha
• HRG1-beta 1
• HRGAneuregulin 1 type IV beta 1a
• MST131
• MSTP131
• NDFheregulin, alpha (45kD, ERBB2 p185-activator)
• neuregulin 1 type IV beta 3
• neuregulin 1
• Neuregulin1
• Neuregulin-1
• NRG1
• pro-neuregulin-1, membrane-bound isoform
• pro-NRG1
• sensory and motor neuron derived factor
• SMDF

Background

Neuregulin 1 (NRG1, isoform HRG-beta2) belongs to the neuregulin/heregulin cytokine family consisting of variants of the four genes NRG1, NRG2, NRG3 and NRG4. More than 15 transmembrane and secreted protein isoforms exist for NRG1, they are generated by alternative promoter usage and mRNA splicing. The different isoforms all contain immunoglobulin (Ig) and epidermal growth factor-like (EGF-like) domains. The EGF-like domain interacts with ErbB3 and ErbB4 tyrosine kinase receptors, resulting in tyrosine phosphorylation and NRG1 signaling. NRG proteins show distinct spatial and temporal expression patterns. These proteins are important for the development of the nervous system and the heart and in the regulation of neurotransmission.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NRG1
• Uniprot:
  • Q02297()
  • Q02297()
  • Q02297()
  • Q02297()