Recombinant Human Neuregulin-1/NRG1 Protein

SDS-Page: Recombinant Human Neuregulin-1/NRG1 Protein [NBP1-99865] - (15%) of purified Neuregulin-1/NRG1 Protein: Lane 1: Marker Lane 2: Neuregulin-1/NRG1 Protein (257-382 aa) - 20 ug

Product Discontinued

Recombinant Human Neuregulin-1/NRG1 Protein Summary

• Description: Recombinant bioactive protein with corresponding amino acids 177-241 of Human Neuregulin-1/NRG1.
  
  Source:E. coli
  
  Accession number:(Q02297)
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab285646)
• Preparation Method: Determined to be >98% pure by SDS-PAGE
• Details of Functionality: The ED50 was determined by the dose-dependent stimulation of the proliferation of human MCF-7 cells is < 0.5 ng/ml, corresponding to a specific activity of > 2 x 106 units/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: NRG1
• Purity: >98%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: The ED50 was determined by the dose-dependent stimulation of the proliferation of human MCF-7 cells is < 0.5 ng/ml, corresponding to a specific activity of > 2 x 10^6 units/mg.
• Theoretical MW: 7.86 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Sterile Filtered lyophilized (freeze-dried) powder.
• Concentration: LYOPH
• Purity: >98%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute in H2O to a concentration of 0.1-1.0 ug/uL. The solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for long-term storage.

Alternate Names for Recombinant Human Neuregulin-1/NRG1 Protein

• ARIA
• GGF2
• GGFglial growth factor
• Heregulin-1
• HGL
• HGLneu differentiation factor
• HRG
• HRG1
• HRG1-alpha
• HRG1-beta 1
• HRGAneuregulin 1 type IV beta 1a
• MST131
• MSTP131
• NDFheregulin, alpha (45kD, ERBB2 p185-activator)
• neuregulin 1 type IV beta 3
• neuregulin 1
• Neuregulin1
• Neuregulin-1
• NRG1
• pro-neuregulin-1, membrane-bound isoform
• pro-NRG1
• sensory and motor neuron derived factor
• SMDF

Background

Neuregulin/Heregulin is a family of structurally related polypeptide growth factors derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 gene. Proteolytic processing of the extraCellular domain of the transmembrane NRG1 isoforms release soluble growth factors. HRG1-Beta 1 contains an Ig domain and an EGF-like domain that is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. This binding induces erb3 and erb4 heterodimerization with erb2, stimulating intrinsic kinase activity, which leads to tyrosine phosphorylation. Although HRG1-Beta 1 biological effects is still unclear, it has been found to promote motility and invasiveness of breast cancer cells which may also involve up-regulation of expression and function of the autocrine motility-promoting factor (AMF). Recombinant human Heregulin-Beta 1 (HRG1-Beta 1) is a 7.5 kDa polypeptide consisting of only the EGF domain of heregulin-Beta 1 (65 amino acid residues).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NRG1