Neuregulin-3/NRG3 Antibody [Unconjugated]

Neuregulin-3/NRG3 was detected in perfusion fixed frozen sections of mouse brain (hippocampus) using Goat Anti-Mouse Neuregulin-3/NRG3 EGF-like Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF753) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell bodies and neuronal processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Neuregulin-3/NRG3 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse NRG3
  Glu284-Pro344
  Accession # O35181
• Specificity: Detects mouse NRG3 EGF-like domain in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NRG3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neuregulin-3/NRG3 Antibody [Unconjugated]

• HRG3
• neuregulin 3 variant 1
• neuregulin 3 variant 10
• neuregulin 3 variant 11
• neuregulin 3 variant 12
• neuregulin 3 variant 13
• neuregulin 3 variant 14
• neuregulin 3 variant 3
• neuregulin 3 variant 4
• neuregulin 3 variant 5
• neuregulin 3 variant 6
• neuregulin 3 variant 7
• neuregulin 3 variant 8
• neuregulin 3 variant 9
• neuregulin 3
• Neuregulin3
• Neuregulin-3
• neuregulin-3-like polypeptide
• NRG3
• pro-neuregulin-3, membrane-bound isoform
• pro-NRG3

Background

The neuregulin (also known as heregulin) cytokine family is comprised of four genes that encode a large number of secreted or membrane-bound isoforms. All family members share an EGF-like domain that interacts with the ErbB family of tyrosine kinase receptors. The NRG1 gene is the most pleiotropic, encoding more than 14 soluble or transmembrane proteins. NRG1 isoforms can be classified into type I (neu differentiation factor, NDF; heregulin, HRG; acetylcholine receptor inducing activity, ARIA), type II (glial growth factor, GGF) and type III (sensory and motor neuron-derived factor, SMDF) isoforms. NRG proteins show distinct spatial and temporal expression patterns and play important roles during development of both the nervous system and the heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neuregulin-3/NRG3 Antibody (AF753)(1)

Publication using AF753

• Wansbury O, Panchal H, James M, Parry S, Ashworth A, Howard B Dynamic expression of Erbb pathway members during early mammary gland morphogenesis. J. Invest. Dermatol., 2007-10-25;128(4):1009-21. 2007-10-25 [PMID: 17960183] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: NRG3
• Uniprot:
  • O35181()