Neuroligin 2/NLGN2 Antibody [Unconjugated]

Western blot shows lysates of human cortex and rat cerebellum tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Rat Neuroligin 2/NLGN2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5645) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for Neuroligin 2/NLGN2 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Neuroligin 2/NLGN2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Neuroligin 2/NLGN2
  Gln15-Ser660
  Accession # EAW90197
• Specificity: Detects human and rat Neuroligin 2/NLGN2 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NLGN2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-3 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neuroligin 2/NLGN2 Antibody [Unconjugated]

• EC 3.1.1
• EC 3.1.1.1
• KIAA1366neuroligin-2
• Neuroligin 2
• NLGN2

Background

Neuroligin 2 (NLGN2) is one of several type I transmembrane Neuroligins that are expressed on neuronal postsynaptic densities. Neuroligins play an important role in synaptic development and function (1). Mature human Neuroligin 2 is a 105 kDa protein that consists of a 663 amino acid (aa) extracellular domain (ECD) with a catalytically inactive cholinesterase-like domain, a 21 aa transmembrane segment, and a 137 aa cytoplasmic tail (2, 3). Within the ECD, human Neuroligin 2 shares 98% aa sequence identity with mouse and rat Neuroligin 2. Alternate splicing generates an isoform with a 17 aa insertion at splice site A within the ECD (2). This recombinant protein does not contain the splice site A insert (-SS A). Neuroligin 2 is expressed on neurons in the brain and also on pancreatic beta cells where it facilitates insulin secretion (2, 4, 5). The -SS A isoform is uniformly expressed among inhibitory and excitatory synapses, while the +SS A isoform is enriched at inhibitory GABAergic synapses (4, 6, 7). Neuroligin 2 clusters at postsynaptic densities in association with other postsynaptic proteins including S-SCAM, PSD-95, gephyrin, and Neuroligin 3 (4, 8 - 10). Synaptic maturation is promoted by the binding of Neuroligin 2 with presynaptic Neurexins, and these interactions are restricted to particular combinations of isoforms of the binding partners (11‑15). Neuroligin 2 interacts with the alpha and beta forms of Neurexin 1, 2, and 3 (14). Its -SS A and +SS A isoforms are bound equally well by Neurexin 1 beta isoforms (-SS4 or +SS4), although only the Neurexin 1 beta +SS4 isoform can induce development of Neuroligin 2‑dependent GABAergic contacts (7, 15).

1. Sudhof, T.C. (2008) Nature 455:903.
2. Ichtchenko, K. et al. (1996) J. Biol. Chem. 271:2676.
3. Koehnke, J. et al. (2008) Proc. Natl. Acad. Sci. 105:1873.
4. Varoqueaux, F. et al. (2004) Eur. J. Cell Biol. 83:449.
5. Suckow, A.T. et al. (2008) Endocrinology 149:6006.
6. Graf, E.R. et al. (2004) Cell 119:1013.
7. Chih, B. et al. (2006) Neuron 51:171.
8. Sumita, K. et al. (2007) J. Neurochem. 100:154.
9. Irie, M. et al. (1997) Science 277:1511.
10. Budreck, E.C. and P. Scheiffele (2008) Eur. J. Neurosci. 26:1738.
11. Chih, B. et al. (2005) Science 307:1324.
12. Chubykin, A.A. et al. (2007) Neuron 54:919.
13. Hines, R.M., et al. (2008) J. Neurosci. 28:6055.
14. Kang, Y. et al. (2008) J. Biol. Chem. 283:2323.
15. Graf, E.R. et al. (2006) J. Neurosci. 26:4256.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NLGN2
• Uniprot:
  • EAW90197()