| Description | The NF-kB / LUCPorter™ reporter cell line can be used for screening of agonists, antagonists or signaling inhibitors related with TLR signaling pathways as well as for studying the NF-kB related signaling pathways.
The NF-kB induction by various Toll-like receptor ligands is shown in Figure In the test, all Toll-like receptor ligands except TLR5 ligand significantly mediated activation of the NF-kB response element which was quantified by luciferase activity (fig. 1). Contents: 3~4 x 10^6 cells Biosafety Level: 2 |
| Immunogen | The NF-kB / LUCPorter™ reporter cell line is a stably transfected RAW 264.7 cell line which expresses an optimized Renilla luciferase reporter gene (RenSP) under the transcriptional control of an NF-kB response element. |
| Target Species | Mouse |
| Specificity | NF-kB LUCPorter™ |
| Selection Agent | Puromycin |
| Growth Properties | Adherent Morphology: Macrophage |
| RCL Type | Luciferase - (LUCPorter™) |
| Host | RAW264.7 |
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| Storage | Store in gas phase of liquid nitrogen. |
| Reconstitution Instructions | Complete Growth Medium: DMEM with 4.5 g/L glucose + 10% FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 100 units/ml penicillin + 0.1 mg/ml streptomycin + 3 ug/ml puromycin. |
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Toll-like receptors in the intestinal epithelial cells By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria ... Read full blog post. |
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The role of MHC Class II RT1B and immune response post brain injury The major histocompatibility complex (MHC) is responsible for binding peptide fragments arising from pathogens in order to display them on the cell surface for recognition from immune cells. Once recognized, the foreign pathogen is typically evade... Read full blog post. |
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NFkB and p62 Both Activate and Regulate Inflammation Nuclear factor kappa-light-chain-enhancer of activated B cells (NFkB) is a protein complex that regulates DNA transcription and is a critical regulator of cell survival. NFkB has long been known as a primer of inflammation, however researchers are ... Read full blog post. |
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Podoplanin (OST8, Glycoprotein (Gp) 36 or 38, Lung Type I Cell Membrane Associated Glycoprotein) Podoplanin is a mucin-type 1 transmembrane glycoprotein found in a wide range of tissues. It appears to be differentially expressed in endothelial cells of lymphatic but not blood vessel origin. In normal skin and kidney, podoplanin co-localizes with ... Read full blog post. |
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Cyclooxygenase 2 (COX2, PGHS2) - I stay with Inflammation! COX2 is an inducible dimeric enzyme belonging to the prostaglandin G/H synthase family that enables cells to respond to growth factors, tumor promoters, and cytokines. Prostaglandins are synthesized through the creation of cyclic endoperoxides from ar... Read full blog post. |
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TRAIL-R1: A Trail of Death and Destruction Cells undergo apoptotic programmed cell death in response to various stimuli. The process is required for morphogenesis, tissue homeostasis, and host defense. Certain cytokines such as tumor necrosis factor (TNF) and Fas ligand signal through death do... Read full blog post. |
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Pulling RANK: Immune Response and Osteoclast Activation by RANKL RANKL is the ligand for the receptor activator of NFkB (RANK) that belongs to the tumor necrosis factor receptor (TNFR) superfamily. RANK overexpression induces the NFkB and c-Jun-terminal kinase (JNK) downstream pathways. This pathway has been studie... Read full blog post. |
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Regulating Immune Response Pathways with IKK beta IKK beta, also known as IKK2, activates the NFkB complex by phosphorylating the NFkB inhibitor, IkBa. Several transcript variants, some protein-coding and some not, have been found for IKKB. The Nuclear Factor-kappa B (NF-kB) family of transcription f... Read full blog post. |
