NG2/MCSP Antibody (LHM 2) - BSA Free

Western Blot: NG2/MCSP Antibody (LHM 2) [NB100-2688] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NG2/MCSP (LHM2) knockout (KO) HeLa cell line. PVDF membrane was probed with 1.0 ug/ml of Mouse Anti-Human NG2/MCSP (LHM2) Polyclonal Antibody (Catalog # NB100-2688) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for NG2/MCSP (LHM2) at approximately 300 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.

Western Blot: NG2/MCSP Antibody (LHM 2) [NB100-2688] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NG2/MCSP (LHM2) knockout (KO) HeLa cell line. PVDF membrane was probed with 1.0 ug/ml of Mouse Anti-Human NG2/MCSP (LHM2) Polyclonal Antibody (Catalog # NB100-2688) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for NG2/MCSP (LHM2) at approximately 300 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.

Western Blot: NG2/MCSP Antibody (LHM 2) [NB100-2688] - Analysis of NG2 expression in HeLa whole cell lysate.

Immunohistochemistry-Paraffin: NG2/MCSP Antibody (LHM 2) [NB100-2688] - Staining of NG2 in human brain using DAB with hematoxylin counterstain.

Flow Cytometry: NG2/MCSP Antibody (LHM 2) [NB100-2688] - A cell surface stain was performed on SK-MEL-28 cells with NG2/MCSP antibody (LHM 2) NB100-2688AF700 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.

Flow Cytometry: NG2/MCSP Antibody (LHM 2) [NB100-2688] - Antibody was tested at 1:400 in MCF7 cells using an Alexa Fluor 488 secondary (shown in green) alongside unstained cells (shown in red).

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) [NB100-2688] - A cell surface stain was performed on U87MG cells with NG2/MCSP antibody (LHM 2) NB100-2688PCP (blue) and a matched isotype control NBP2-27287PCP (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Peridinin-Chlorophyll-Protein complex.

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) [NB100-2688] - A cell surface stain was performed on U87MG cells with NG2/MCSP antibody (LHM 2) NB100-2688AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow (Cell Surface): NG2/MCSP Antibody (LHM 2) [NB100-2688] - A surface stain was performed on A375 Cells with NG2/MCSP (LHM2) antibody NB100-2688 (blue) and a matched isotype control NBP1-43319 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, IP, KO
• Clone: LHM 2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

NG2/MCSP Antibody (LHM 2) - BSA Free Summary

• Immunogen: A 375P cells crude extract
• Localization: Cell Membrane
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CSPG4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow (Cell Surface) 1 - 2.5 ug/mL
  • Flow Cytometry 1 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10 - 1:100
  • Knockout Validated
  • Western Blot 1:1000
• Application Notes: In WB a band can be seen at ~300 kDa.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for NG2/MCSP Antibody (LHM 2) - BSA Free

• AN2
• chondroitin sulfate proteoglycan 4 (melanoma-associated)
• chondroitin sulfate proteoglycan 4
• CSPG4
• EC 3.6.3
• HMW-MAA
• MCSP
• MCSPChondroitin sulfate proteoglycan NG2
• MCSPG
• MCSPGMelanoma chondroitin sulfate proteoglycan
• MEL-CSPG
• MSK16Melanoma-associated chondroitin sulfate proteoglycan
• NG2
• NG2EC 2.7.8

Background

NG2 (Neuron Glial Antigen 2), also known as MCSP, and CSPG4 is a chondroitin surface proteoglycan. Chondroitin surface proteoglycans play a role in stabilizing cell-substratum interactions during early events of cell spreading. At large, proteoglycans are important for cell proliferation and migration, and may act as signal transducers through C-terminal cytoplasmic binding to scaffolding or signaling proteins. The NG2 glycoprotein is a type I membrane protein expressed in the developing and adult central nervous system (CNS) by subpopulations of glia including oligodendroglial precursor cells (OPCs), and in the developing CNS additionally by pericytes. NG2 is located at the apical cell membrane, however it has also been shown to localize to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Research has found that NG2 may inhibit neuronal growth (PMC1571583), and also acts as a proapoptotic receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NG2/MCSP Antibody (NB100-2688)(13)

Publications using NB100-2688

• Koivunotko E, Snirvi J, Merivaara A Et al. Angiogenic Potential of Human Adipose-Derived Mesenchymal Stromal Cells in Nanofibrillated Cellulose Hydrogel Biomedicines 2022-10-27 [PMID: 36289846] (WB, Human)
  
  Details:
  Citation using the Azide and BSA Free version of this antibody.
• Lin Z, Zhang X, Fritch MR Et al. Engineering pre-vascularized bone-like tissue from human mesenchymal stem cells through simulating endochondral ossification Biomaterials 2022-03-08 [PMID: 35259584] (IHC-P, Human)
  
  Details:
  Citation using the Alexa Fluor 594 version of this antibody.
• Liu HY, Koch C, Haller A Et al. Evaluation of Microfluidic Ceiling Designs for the Capture of Circulating Tumor Cells on a Microarray Platform Adv Biosyst 2020-04-16 [PMID: 32293134]
  
  Details:
  Citation using the PE version of this antibody.
• Arrigoni C, Bongio M, Talo G et al. Rational Design of Prevascularized Large 3D Tissue Constructs Using Computational Simulations and Biofabrication of Geometrically Controlled Microvessels. Adv Healthc Mater. 2016-05-18 [PMID: 27191352] (ICC/IF, Human)
• Stoyanov D The Role of Transcription Factor ZBTB20 in the Development of Interneurons in the Neocortex of Mice Thesis 2023-01-01
• Dey D, Tyagi S, Shrivastava V et al. Using human fetal neural stem cells to elucidate the role of the JAK-STAT cell signaling pathway in oligodendrocyte differentiation in vitro Research Square 2023-11-01 [PMID: 38227271] (ICC/IF, Human)
• Monica Nanni, Dominic Rütsche, Curdin Bächler, Luca Pontiggia, Agnes S. Klar, Ueli Moehrlen, Thomas Biedermann CD146 expression profile in human skin and pre-vascularized dermo-epidermal skin substitutes in vivo Journal of Biological Engineering 2023-01-31 [PMID: 36721239]
• Li X, Guo W, Zha K et al. Enrichment of CD146+ Adipose-Derived Stem Cells in Combination with Articular Cartilage Extracellular Matrix Scaffold Promotes Cartilage Regeneration Theranostics 2019-07-09 [PMID: 31410204] (WB, Human)
• Kinney HC, Haynes RL, Xu G, Andiman SE, Folkerth RD, Sleeper LA, Volpe JJ. Neuron deficit in the white matter and subplate in periventricular leukomalacia. Ann Neurol;71(3):397-406. 2012-03-01 [PMID: 22451205] (IF/IHC, ICC/IF, Human)
• Pierce GL, Donato AJ, LaRocca TJ, Eskurza I, Silver AE, Seals DR. Habitually exercising older men do not demonstrate age-associated vascular endothelial oxidative stress. Aging Cell;10(6):1032-7. 2011-12-01 [PMID: 21943306] (ICC/IF, Human)
• Wang Y-C, Nakagawa M, Garitaonandia I et al. Specific lectin biomarkers for isolation of human pluripotent stem cells identified through array-based glycomic analysis. Cell Research. 2011-09-06 [PMID: 21894191]
• Kose O, Kutulola AO, Odell EW, Waseem A. Changes in the expression of stem cell markers in oral lichen planus and hyperkeratotic lesions. J Oral Sci. 2007-06-01 [PMID: 17634726] (IF/IHC, Human)
• Legg J, Jensen UB, Broad S et al. Role of melanoma chondroitin sulphate proteoglycan in patterning stem cells in human interfollicular epidermis. Development. 2003-12-01 [PMID: 14573520] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: CSPG4
• Entrez:
  • 1464(Human)
• Uniprot:
  • Q6UVK1()