Nicalin Antibody

Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, CEM human T-lymphoblastoid cell line, Huh-7 human hepatoma cell line, Neuro-2A mouse neuroblastoma cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/ Mouse/Rat Nicalin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7686) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Nicalin at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Nicalin Antibody Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Nicalin
  Met1-Ala522, predicted
  Accession # Q969V3
• Specificity: Detects human, mouse, and rat Nicalin in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: NCLN
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nicalin Antibody

• Ncl-1
• NCLN
• NET59
• nicalin homolog (zebrafish)
• nicalin homolog (zebrefish)
• Nicalin
• Nicastrin-like protein

Background

Nicalin (NICAstrin-Like proteIN) is a 60-62 kDa member of the nicastrin family of proteins. It is an ER-embedded transmembrane protein that is found in skeletal and cardiac muscle, hepatocytes and neurons. Nicalin interacts with 22 kDa TMEM147 and 130 kDa NOMO1 to form a 200-220 kDa complex that antagonizes Nodal signaling. Mature human nicalin is a 521 amino acid (aa) type I transmembrane glycoprotein. It contains a 480 aa luminal/extracellular region (aa 43-522) plus a 20 aa cytoplasmic domain (aa 544-563). The luminal region possesses one likely inactive M28 Zn-peptidase-like domain (aa 130-429). Four potential isoform variants are reported. One shows an alternative start site at Met75, a second contains a seven aa insertion after Gln543, a third contains a 102 aa substitution for aa 162-563, and a fourth shows a deletion of Gln446. Over aa 1-522, human nicalin shares 95% aa sequence identity with mouse nicalin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCLN
• Uniprot:
  • Q969V3(Human)