Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8)

Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells and NNMT-Knockout HeLa cells were separated by SDS-PAGE and immunoblotted with anti-NNMT monoclonal antibody (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

Immunocytochemistry/Immunofluorescence: NNMT Antibody (3D8) [NBP2-00537] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NNMT.

Immunohistochemistry-Paraffin: NNMT Antibody (3D8) [NBP2-00537] - Staining of paraffin-embedded Human prostate tissue using anti-NNMT mouse monoclonal antibody.

Flow Cytometry: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) [NBP2-00537] - Analysis of Jurkat cells, using anti-NNMT antibody (Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: NNMT Antibody (3D8) [NBP2-00537] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NNMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NNMT.

Western Blot: NNMT Antibody (3D8) [NBP2-00537] Analysis of extracts (35ug) from 9 different cell lines by using anti-NNMT monoclonal antibody.

Immunohistochemistry-Paraffin: NNMT Antibody (3D8) [NBP2-00537] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NNMT mouse monoclonal antibody.

Immunohistochemistry-Paraffin: NNMT Antibody (3D8) [NBP2-00537] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NNMT mouse monoclonal antibody.

Immunohistochemistry-Paraffin: NNMT Antibody (3D8) [NBP2-00537] - Staining of paraffin-embedded Human endometrium tissue using anti-NNMT mouse monoclonal antibody.

Immunohistochemistry-Paraffin: NNMT Antibody (3D8) [NBP2-00537] - Staining of paraffin-embedded Human liver tissue using anti-NNMT mouse monoclonal antibody.

Immunohistochemistry-Paraffin: NNMT Antibody (3D8) [NBP2-00537] - Staining of paraffin-embedded Human pancreas tissue using anti-NNMT mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, KO
• Clone: OTI3D8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) Summary

• Immunogen: Full length human recombinant protein of human NNMT(NP_006160) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NNMT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Knockout Validated
  • Western Blot 1:500-2000
• Theoretical MW: 29.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8)

• EC 2.1.1.1
• Nicotinamide NMethyltransferase
• Nicotinamide N-Methyltransferase
• NNMT

Background

N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nicotinamide N-Methyltransferase/NNMT Antibody (NBP2-00537)(6)

Publications using NBP2-00537

• Dolcen DN The Role of Nicotinamide N-Methyltransferase in Triple-Negative Breast Cancer Gene Expression and in vivo Metastatic Potential Thesis 2022-01-01 (WB, Human)
• Chen C, Wang X, Huang X et al. Nicotinamide N-methyltransferase: a potential biomarker for worse prognosis in gastric carcinoma. Am J Cancer Res 2016-02-15 [PMID: 27152242] (WB)
• Xu Y, Liu P, Zheng DH et al. Expression profile and prognostic value of NNMT in patients with pancreatic cancer. Oncotarget 2016-04-12 [PMID: 26942567]
• AKAR S, HARMANKAYA I Nicotinamide N-methyltransferase Expression and its Association with Phospho-Akt, p53 Expression and Survival in High Grade Endometrial Cancer Turk J Med Sci Sep 22 2019
• Harmankaya İ, Akar S, Ugras S et al. Nicotinamide N-methyltransferase overexpression may be associated with poor prognosis in ovarian cancer J Obstet Gynaecol 2020-04-14 [PMID: 32285726] (IF/IHC, Human)
• Akar S, Harmankaya I et al. Nicotinamide N-methyltransferase expression and its association with phospho-Akt, p53 expression, and survival in high-grade endometrial cancer Turk J Med Sci 2019-10-24 [PMID: 31652035] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: NNMT
• Entrez:
  • 4837(Human)